Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle

The data presented are quantitative polymerase chain reaction (qPCR) read outs from antimicrobial resistance gene (AMRG) assays and associated metadata from this project. In this dataset, the mean gene copy numbers per microlitre of DNA extract are shown. The data were collected from faecal and environmental samples which were obtained from a single British commercial pig unit. The former were collected from the sow housing barn, pig growing houses and slurry tanks within the farm unit and the latter were obtained through random stratified sampling of the farm and the surrounding land. These samples were taken from what will be referred to as the 'main study'. A further study was carried out to obtain samples after a partial depopulation which took place on this farm. Faecal samples were obtained from the sow housing barn, pig growing houses and slurry tanks and will be referred to as the 'depopulation (depop) study'. For the main study, the samples were collected between October 19th 2016 and April 5th 2017. For the depop study, the samples were collected between 19th June 2017 and 13th November 2017. The data associated with all samples were generated between August 1st 2017 and May 1st 2018. Full details about this dataset can be found at https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2018-08-10

Identifier: https://catalogue.ceh.ac.uk/id/e548dc5d-49e3-467d-9435-c199da40e7be

Identifier: doi: / 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be

Other citation details: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be

The Roslin Institute - Dr. Jolinda Pollock

SRUC/The Roslin Institute - Pollock, J.

R(D)SVS/The Roslin Institute - Corbishley, A.

SRUC - Hutchings, M.R.

The Roslin Institute - Gally, D.L.

NERC Environmental Information Data Centre

NERC EDS Environmental Information Data Centre

University of Edinburgh

GEMET - INSPIRE themes, version 1.0 ()

Soil
Agricultural and Aquaculture Facilities
Human Health and Safety

Keywords

Agriculture

Limitations on Public Access: otherRestrictions

Other constraints: no limitations

Use constraints: otherRestrictions

Other constraints: This resource is made available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: Â© University of Edinburgh

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be

Spatial representation type: textTable

Topic category

Biota
Farming

Begin date: 2016-10-19

End date: 2017-04-05

Code: OSGB 1936 / British National Grid

Distribution Information

Data format

Comma-separated values (CSV) ()

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Resource Locator: Download the data
Download a copy of this data

Resource Locator: Supporting information
Supporting information available to assist in re-use of this dataset

Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Faecal and soil samples were collected from the study farm weekly using spooned universal tubes and were stored at -20Â°C, batch transported on dry ice to the laboratory where they were stored at -80Â°C until DNA extraction was carried out. DNA extracts were quantified and assessed using a NanoDrop spectrophotometer and split into aliquots for storage. DNA extracts were then screened in triplicate by qPCR and the number of gene copies assessed through the use of a standard curve generated by the inclusion of standards with known copy numbers of each target gene. Non-template controls were also included to ensure that non-specific binding or contamination were not occurring. The qPCR machine software (i.e. MxPro) was then used to calculate the number of gene copies in each of the collected samples and the mean was calculated using the three replicates. These values are included in the data set provided.

Metadata

File identifier: e548dc5d-49e3-467d-9435-c199da40e7be XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-05-18T13:09:33

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

Overviews

Publishing Body

Access the portal

Full access to the portal and metadata.

Associated resources (if any)

Not available

Default