This dataset presents the results of an initial sampling exercise conducted at a terrestrial site in northwest England in summer 2010. The following samples of terrestrial Reference Animals and Plants (RAPs) were obtained from an area of circa 0.4 km squared: Molinia caerulea (ICRP RAP Wild Grass defined as Poaceae); Picea sitchensis (ICRP RAP Pine Tree defined as Pinaceae); Apis spp., Bombus spp., Nomada spp. (ICRP RAP Bee defined as Apidea); Apodemus sylvaticus (ICRP RAP Rat defined as Muridae); Earthworms (species in the Family Lumbricidae as defined for the ICRP RAP Earthworm); Deer (belonging to the Family Anatidae (i.e. the ICRP RAP Deer). Soil samples were also collected from throughout the sampling area. All samples were analysed for multiple elements using ICP-MS/ICP-OES and most for gamma-emitting radionuclides. Results have been used to derive biota-soil concentration ratios. The ICRP have published their framework for radiation protection of the environment (ICRP Publication 108). This describes the use of RAPs as the basis for their framework. The RAPs are generalised to the taxonomic level of Family. Publication 108 presented dose coefficient values for the selected RAPs and also reviewed data on the effects of ionising radiation to suggest Derived Consideration Reference Levels for each RAP. In summer 2010 the ICRP released a further report on their protection framework for consultation. This report presented transfer parameter values (organism-media concentration ratios) for Reference Animals and Plants. The report also raised the possibility of identifying a series of sites where samples of each Reference Animal and Plant, and their different lifestages, could be collected and analysed. It was suggested that the resultant data would constitute a set of reference values analogous to approaches used by the ICRP for human radiological protection. Full details about this dataset can be found at https://doi.org/10.5285/e40b53d4-6699-4557-bd55-10d196ece9ea