Antibiotic susceptibility tests and resistance genes in Escherichia coli from humans, poultry and environmental samples in urban and rural Bangladesh (2017)

Antibiotic susceptibility tests are presented as the zone of inhibition using the disc-diffusion method, and categorized as resistant, intermediate or susceptible. DNA samples from antibiotic-resistant bacteria were analysed for the presence or absence of resistance genes using polymerase chain reaction (PCR). Laboratory analyses were conducted by trained staff at the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b). The aim of the study was to identify the antibiotic-susceptibility profiles and resistance genes of bacteria (Escherichia coli) obtained from humans, poultry and the environment. Bacterial isolates previously identified with resistance to third-generation cephalosporins or carbapenems were included in the analysis. Bacterial samples originated from rural households and poultry farms (broiler chickens) in Mirzapur, Tangail district; and urban food markets in Dhaka city, Bangladesh. Environmental samples included surface water, water supply, wastewater, soil, animal faeces (poultry and cattle) and solid waste. The survey was part of a wider research project, Spatial and Temporal Dynamics of Antimicrobial Resistance Transmission from the Outdoor Environment to Humans in Urban and Rural Bangladesh. The research was funded by NERC/BBSRC/MRC on behalf of the Antimicrobial Resistance Cross-Council Initiative award NE/N019555/1. Full details about this dataset can be found at https://doi.org/10.5285/dda6dd55-f955-4dd5-bc03-b07cc8548a3d

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2019-02-11

Dataset Reference Date (): 2018-12-15

Identifier: https://catalogue.ceh.ac.uk/id/dda6dd55-f955-4dd5-bc03-b07cc8548a3d

Identifier: doi: / 10.5285/dda6dd55-f955-4dd5-bc03-b07cc8548a3d

Other citation details: Rousham, E.K., Islam, M.A., Unicomb, L., Asaduzzaman, M., Islam, R., Amin, B., Mozmader, T.I.M.A., Hossain, M.I., Rahman, M. (2019). Antibiotic susceptibility tests and resistance genes in Escherichia coli from humans, poultry and environmental samples in urban and rural Bangladesh (2017). NERC Environmental Information Data Centre 10.5285/dda6dd55-f955-4dd5-bc03-b07cc8548a3d

Loughborough University - Dr Emily Rousham

Loughborough University - Rousham, E.K.

International Centre for Diarrhoeal Disease Research, Bangladesh - Islam, M.A.

International Centre for Diarrhoeal Disease Research, Bangladesh - Unicomb, L.

International Centre for Diarrhoeal Disease Research, Bangladesh - Asaduzzaman, M.

International Centre for Diarrhoeal Disease Research, Bangladesh - Islam, R.

International Centre for Diarrhoeal Disease Research, Bangladesh - Amin, B.

International Centre for Diarrhoeal Disease Research, Bangladesh - Mozmader, T.I.M.A.

International Centre for Diarrhoeal Disease Research, Bangladesh - Hossain, M.I.

International Centre for Diarrhoeal Disease Research, Bangladesh - Rahman, M.

NERC EDS Environmental Information Data Centre

NERC Environmental Information Data Centre

Maintenance and update frequency: notPlanned

Wikidata

Escherichia coli

Keywords

antibiotic susceptibility tests
antimicrobial resistance
infectious disease
microbiology
bacterial counts
carbapenem
third-generation cephalosporin
DNA

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Other constraints: © Loughborough University

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Other constraints: © International Centre for Diarrhoeal Disease Research, Bangladesh

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Other constraints: This resource is made available under the terms of the Open Government Licence

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Other constraints: © Natural Environment Research Council

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Other constraints: If you reuse this data, you should cite: Rousham, E.K., Islam, M.A., Unicomb, L., Asaduzzaman, M., Islam, R., Amin, B., Mozmader, T.I.M.A., Hossain, M.I., Rahman, M. (2019). Antibiotic susceptibility tests and resistance genes in Escherichia coli from humans, poultry and environmental samples in urban and rural Bangladesh (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/dda6dd55-f955-4dd5-bc03-b07cc8548a3d

Spatial representation type: textTable

Topic category

Health

Begin date: 2017-02-01

End date: 2018-10-31

Code: WGS 84

Distribution Information

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Comma-separated values (CSV) ()

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Report

Dataset Reference Date (): 2010-12-08

Statement: Water (drinking, stream, waste and pond), soil and faecal samples were collected from farms and urban markets. Laboratory analysis of bacterial samples took place at International Centre for Diarrhoeal Disease Research , Dhaka, Bangladesh. Laboratory results were recorded on paper-based forms or generated from automated readings, entered into Excel then converted to .csv files for ingestion into the Environmental Information Data Centre. Reference organisms and Clinical and Laboratory Standard Institute (CLSI) guidelines were followed.

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File identifier: dda6dd55-f955-4dd5-bc03-b07cc8548a3d XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2022-05-18T12:30:03

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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