d6c787ec-146c-461b-b8a2-e0251259036c English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:26:37 UK GEMINI 2.3 WGS 84 / Pseudo-Mercator Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019 2022-08-31 publication https://catalogue.ceh.ac.uk/id/d6c787ec-146c-461b-b8a2-e0251259036c 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c doi: Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c Fungal community DNA was sequenced from soils in northern, subarctic Sweden. Soils were sampled from treeline mountain birch forest and stands of tundra willow that were wither ‘girdled’ (connection between leaves and roots cut by disrupting the phloem transport) or ‘control’ (no treatment), 0, 1 and 2 years after the treatment was applied. Full details about this dataset can be found at https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c Parker, T.C. James Hutton Institute Thomas.parker@hutton.ac.uk https://orcid.org/0000-0002-3648-5316 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Chomel, M. University of Manchester mathilde.chomel@manchester.ac.uk https://orcid.org/0000-0001-5110-2355 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Clemmensen, K.E. Swedish University of Agricultural Sciences karina.clemmensen@slu.se https://orcid.org/0000-0002-9627-6428 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Friggens, N.L. University of Exeter N.Lindstrom-Friggens@exeter.ac.uk https://orcid.org/0000-0002-5536-6312 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Hartley, I.P. University of Exeter I.Hartley@exeter.ac.uk https://orcid.org/0000-0002-9183-6617 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Johnson, D. University of Manchester david.johnson-2@manchester.ac.uk https://orcid.org/0000-0003-2299-2525 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Krab, E.J. Swedish University of Agricultural Sciences eveline.krab@slu.se https://orcid.org/0000-0001-8262-0198 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Lindahl, B.D. Swedish University of Agricultural Sciences Bjorn.Lindahl@slu.se https://orcid.org/0000-0002-3384-4547 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Street, L.E. University of Edinburgh Lorna.Street@ed.ac.uk https://orcid.org/0000-0001-9570-7479 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Subke, J.-. University of Stirling jens-arne.subke@stir.ac.uk https://orcid.org/0000-0001-9244-639X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Wookey, P.A. University of Stirling philip.wookey1@stir.ac.uk https://orcid.org/0000-0001-5957-6424 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Parker, T. James Hutton Institute Thomas.parker@hutton.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian University of Stirling jens-arne.subke@stir.ac.uk owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication tundra soil fungus tree GEMET - Concepts, version 4.1.3 2019-01-28 creation Sweden Subarctic Treeline Girdling otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c textTable 3 English utf8 biota environment 2017-08-03 2019-08-05 12.017 22.395 55.106 71.663 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/d6c787ec-146c-461b-b8a2-e0251259036c Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/d6c787ec-146c-461b-b8a2-e0251259036c.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The organic soil horizon (O horizon) was sampled on 3rd August 2017, 1st August 2018 and 5th August 2019. For each plot (22 in total), nine 3.8 cm diameter soil cores were collected; in the birch plots, cores were taken in a grid across a 3 x 3 m central area (corresponding to the central area in which soil CO2 efflux, root and mycelium production and soil fauna measurements were taken; (Parker et al., 2020). In the willow plots, the nine cores were distributed evenly across the plot area within the trenched perimeter. The uppermost litter layer, as well as the mineral horizons underlying the organic horizon (clearly identifiable at these sites), were removed from cores immediately after coring. The nine cores from each plot were homogenised and pooled within 6 hours of sampling. DNA was extracted from a 50 mg sub-sample and ITS2 markers were amplified by PCR. We used a community metabarcoding approach on the pooled DNA samples to identify distinct taxonomic groups at species level where possible. Copy numbers of the fungal ITS2 region were estimated by quantitative PCR (qPCR)