cf3133fb-0bd5-48e5-a89c-d464bd5b90a3 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2023-12-12 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures - VERSION 2.0 2023-12-12 creation 2023-12-12 revision 2023-12-12 publication 2023-12-12 released 2.0 https://doi.org/10.5285/cf3133fb-0bd5-48e5-a89c-d464bd5b90a3 doi GB/NERC/BAS/PDC/01807 https://data.bas.ac.uk/ Please cite this item as: Newsham, K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures - VERSION 2.0 (Version 2.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/cf3133fb-0bd5-48e5-a89c-d464bd5b90a3 Data on the growth rates and specific cellulase activities of 10 isolates of the fungus Pseudogymnoascus roseus from sub- and maritime Antarctica, exposed in vitro to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius and their corresponding mean constant temperatures of 9.0 degrees Celsius, 10.7, 12.0 or 13.1 degrees Celsius, respectively, and constant temperatures of 2 degrees Celsius, 5.5 degrees Celsius, 8.5 degrees Celsius, 15.0 degrees Celsius, 18.0 degrees Celsius, 21.0 degrees Celsius and 24.0 degrees Celsius. Funding: UK Natural Environment Research Council (core funding to the British Antarctic Survey). No credit. completed Newsham, Kevin British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Fungi theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctic heatwaves beta-glucosidase cellulase fluctuating temperature growth rate psychrotrophy soil fungi theme Lewis Pass, South Georgia The Backslope, Signy Island Walton Terraces, Leonie Island Mars Oasis, Alexander Island place Habitats and biotopes theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions Data released under Open Government Licence V3.0: Use the following citation: Newsham, K. K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3EF187B8-3471-42AB-BC5D-D7E5E6F5D575 otherRestrictions No restrictions apply. url url crossReference doi doi crossReference textTable eng utf8 biota -68.35778 -36.49833 -71.87833 -54.26472 2021-09-01 2023-03-31 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor bytes 1 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=cf3133fb-0bd5-48e5-a89c-d464bd5b90a3 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: A total of 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3). They were maintained on half-strength potato dextrose medium. Three experiments were conducted. In the first, each isolate was inoculated onto soil extract medium in eight Petri dishes, which were incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony fronts were then marked on dish reverses with a fine blade at × 10-15 magnification (accuracy c. 100 micrometers) and four dishes inoculated with each isolate were transferred to cabinets fluctuating daily from 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius. The other four dishes inoculated with each isolate were transferred back to the cabinets running at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius. Note that the constant temperatures were the means of the fluctuating temperatures (e.g., 9.0 degrees Celsius was the mean of the temperatures fluctuating from 2-15 degrees Celsius). Colony fronts were marked on dish reverses 24 hours and 48 hours later, and mean growth rate (mm / day) was calculated. In the second experiment, soil extract medium in eight Petri dishes was again inoculated with each of the 10 isolates, which were again incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony radii were then measured twice at right angles to each other at × 10-15 magnification. Four dishes per isolate that had been incubated at each of these temperatures were then placed into cabinets set to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius or 2-24 degrees Celsius, respectively, and the remaining four dishes per isolate were replaced in the cabinets set to constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius. Colony radii were measured every 3-5 days over the next 21 days and mean growth rates (mm / day) were calculated by regressing colony radii against time. After 21 d, extracellular beta-glucosidase (cellulase) was extracted from colonies in 10 mM phosphate buffer. Protein concentrations in the buffer were measured using a Micro BCA Protein Assay (Thermofisher). Cellulase activity was measured and expressed as nmol of p-nitrophenol released from 4-nitrophenyl-beta-D-glucopyranoside / min / microgammes protein (Misiak et al. 2021, doi: 10.1111/gcb.15456). In the third experiment, the 10 isolates were each inoculated onto soil extract medium in four Petri dishes, which were incubated at constant temperatures of 2.0 degrees Celsius, 5.5 degrees Celsius, 8.5 degrees Celsius, 15.0 degrees Celsius, 18.0 degrees Celsius, 21.0 degrees Celsius and 24.0 degrees Celsius for one week. Colony fronts were then marked at × 10-15 magnification on dish reverses as described above. The fronts were marked again after 24 hours and 48 hours and radial extension rate (mm / day), averaged over 48 hours, was calculated. This dataset was updated to include additional data from the third experiment described above and to update the metadata accordingly. Data collection: Micro BCA Protein Assay (Thermofisher) Data quality: Data have not been cleaned. Data losses, indicated by MD (missing data) were caused by laboratory errors. Note that the accuracy of growth rate data measured at 12 degrees Celsius and 2-21 degrees Celsius over 0-48 hours was lower than for other growth rate data. asNeeded completed