Microbial DNA extraction and PCR from core samples SSK111456 and SSK111457 from UKGEOS Glasgow Observatory borehole GGC01

Loan IDA number IDA271576. Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent PCR from shale and sandstone core samples collected and preserved for microbiology (shale SSK111456 and sandstone SSK111457) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey.

Date (Creation): 2021-06-22

Identifier: http://data.bgs.ac.uk/id/dataHolding/13607775

Originator

University of Bristol - Katie Edwards ( PhD Student School of Earth Sciences )

Wills Memorial Building, Queens Road, Clifton , Bristol , BS8 1RJ ,

Distributor

British Geological Survey - Enquiries

United Kingdom

Point of contact

British Geological Survey - Enquiries

United Kingdom

Maintenance and update frequency: notApplicable notApplicable

GEMET - INSPIRE themes, version 1.0

Geology

BGS Thesaurus of Geosciences

Geomicrobiology
Sandstone
Shale

dataCentre

Keywords

NERC_DDC

Access constraints: otherRestrictions Other restrictions

Other constraints: licenceOGL

Other constraints: Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Use constraints: otherRestrictions Other restrictions

Other constraints: The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints: Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Spatial representation type: textTable Text, table

Metadata language: EnglishEnglish

Topic category

Geoscientific information

Geographic identifier

Glasgow [id=1298677]

Date (Revision): 2002

Begin date: 2020-03-01

End date: 2020-08-31

Unique resource identifier: OSGB 1936 / British National Grid (EPSG::27700)

Distribution format

Microsoft Excel ( witheld )

Distributor

British Geological Survey - Enquiries

United Kingdom

OnLine resource: Data ( WWW:DOWNLOAD-1.0-http--download )

Hierarchy level: dataset Dataset

Other: dataset

Conformance result

Date (Publication): 2011

Explanation: See the referenced specification

Pass: No

Conformance result

Date (Publication): 2010-12-08

Explanation: See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass: No

Statement: Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.