b6900166-ded6-4f7a-8734-484b6f77b2f1 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:21:04 UK GEMINI 2.3 WGS 84 Comparative transcriptomics of a complex of four European pine species 2014-10-10 publication 1412239567948 CEH:EIDC: https://catalogue.ceh.ac.uk/id/b6900166-ded6-4f7a-8734-484b6f77b2f1 10.5285/b6900166-ded6-4f7a-8734-484b6f77b2f1 doi: Wachowiak, W., Trivedi, U., Telford, A., Cavers, S. (2014). Comparative transcriptomics of a complex of four European pine species. NERC Environmental Information Data Centre 10.5285/b6900166-ded6-4f7a-8734-484b6f77b2f1 Data from whole transcriptome sequencing of the four European pine species - Pinus sylvestris (Scots pine), P. mugo (Dwarf mountain pine), P. uliginosa (Mountain pine) and P. uncinata (Peat-bog pine). Those data include 1) transcriptome sequence of a reference Scots pine sample generated using Illumina paired-end sequencing technology and 2) information about single nucleotide polymorphisms (SNPs) in 17 samples collected across the European range, compared to the reference Scots pine sample. These genomic resources were produced to advance the study of the genetic basis of adaptive variation and speciation in pines. Full details about this dataset can be found at https://doi.org/10.5285/b6900166-ded6-4f7a-8734-484b6f77b2f1 Wachowiak, W. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Trivedi, U. The University of Edinburgh enquiries@ceh.ac.uk author Telford, A. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Cavers, S. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-2139-9236 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Wachowiak, W. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner notPlanned Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Pinus sylvestris Pinus mugo Pinus uliginosa Pinus uncinata Wikidata 2012-10-29 creation Evolutionary ecology Europe otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © UK Centre for Ecology & Hydrology otherRestrictions If you reuse this data, you should cite: Wachowiak, W., Trivedi, U., Telford, A., Cavers, S. (2014). Comparative transcriptomics of a complex of four European pine species. NERC Environmental Information Data Centre https://doi.org/10.5285/b6900166-ded6-4f7a-8734-484b6f77b2f1 textTable English utf8 biota 2006-01-01 2008-12-31 -5.5 29.5 40 61.5 Variant Call Format (VCF) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/b6900166-ded6-4f7a-8734-484b6f77b2f1 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/b6900166-ded6-4f7a-8734-484b6f77b2f1.zip Supporting Information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 1. Lineage of the reference transcriptome sequence of Scots pine samples from Glen Tanar (Scotland). Total Ribonucleic acid (RNA) was extracted from needles using Spectrum (TM) Plant Total RNA Kit (Sigma). A template complementary DNA (cDNA) library was prepared using ®TruSeq RNA Sample Preparation Kit (Illumina). The cDNA library was sequenced using Illumina HiSeq 2000 platform at Edinburgh Genomics, the University of Edinburgh, Scotland (Illumina, San Diego, California). 100 base, paired-end reads were generated and assembled de novo into contigs using Trinity (version r2012-06-08). The reference transcript sequence contains 40798 contigs in total. 2. The single nucleotide polymorphism (SNP) data file was prepared by aligning sixteen samples to the reference, a sample set that included P. sylvestris (four samples), P. mugo (five samples), P. uncinata (four samples) and P. uliginosa (three samples). Samples collected between 2006 and 2010.