Intra-crystalline protein degradation dating of proboscidean enamel from the UK (NERC Grant NE/K500987/1)

The concentration of both L and D amino acid isomers was determined by HPLC (High-performance liquid chromatography) for multiple amino acids. 30 samples of proboscidean enamel were taken from the collections at BGS. Samples of enamel (~30 mg) was removed from the teeth of proboscideans using a small electric drill. All of the samples were from UK sites dating to the Quaternary. These samples were used to build a regional geochronology based on the extent of racemisation of amino acids from the intra-crystalline fraction (Dickinson, 2018). Thesis http://etheses.whiterose.ac.uk/22261. A published paper 'A new method for enamel amino acid racemization dating: A closed system approach' is also available; https://doi.org/10.1016/j.quageo.2018.11.005

Date (Creation): 2020-11-27

Identifier: http://data.bgs.ac.uk/id/dataHolding/13607694

Originator

University of York - Marc Dickinson ( Chemistry Department )

York , YO10 5DD ,

Distributor

British Geological Survey - Enquiries

Environmental Science Centre, Nicker Hill, Keyworth , NOTTINGHAM , NOTTINGHAMSHIRE , NG12 5GG , United Kingdom

0115 936 3143
0115 936 3276

Point of contact

British Geological Survey - Enquiries

Environmental Science Centre, Nicker Hill, Keyworth , NOTTINGHAM , NOTTINGHAMSHIRE , NG12 5GG , United Kingdom

0115 936 3143
0115 936 3276

Maintenance and update frequency: notApplicable notApplicable

GEMET - INSPIRE themes, version 1.0

Geology

BGS Thesaurus of Geosciences

NGDC Deposited Data
Age determination methods
Racemization dating
Geochronology

dataCentre

NGDC Deposited Data

Keywords

NERC_DDC

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Other constraints: licenceOGL

Other constraints: Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Use constraints: otherRestrictions Other restrictions

Other constraints: The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints: Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Spatial representation type: textTable Text, table

Metadata language: EnglishEnglish

Topic category

Geoscientific information

Geographic identifier

ENG

Date (Revision): 2009

Geographic identifier

ENGLAND [id=150000]

Date (Creation): 1979

Begin date: 2017-05-08

End date: 2018-07-01

Unique resource identifier: OSGB 1936 / British National Grid (EPSG::27700)

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Images (JPEG) ()
MS Excel ()

Distributor

British Geological Survey - Enquiries

Environmental Science Centre, Nicker Hill, Keyworth , NOTTINGHAM , NOTTINGHAMSHIRE , NG12 5GG , United Kingdom

0115 936 3143
0115 936 3276

OnLine resource: http://etheses.whiterose.ac.uk/22261

OnLine resource: https://doi.org/10.1016/j.quageo.2018.11.005

OnLine resource: Data

Hierarchy level: dataset Dataset

Other: dataset

Conformance result

Date (Publication): 2011

Explanation: See the referenced specification

Pass: No

Conformance result

Date (Publication): 2010-12-08

Explanation: See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass: No

Statement: Samples of enamel (~30 mg) were powdered and exposed to NaOCl (12%) for 72 h. The NaOCl was removed and the powdered enamel was washed 5X with water and 1X with MeOH. Powdered enamel samples were split into 2 fractions. One sample was treated for their free amino acid (FAA) content and the other their total hydrolysable amino acid (THAA) content. THAA samples were dissolved in HCl (7M) and heated at 110 ̊C for 24 h. THAA samples were re-dissolved in HCl (1 M, 20 μLmg−1) and FAA samples were demineralised in HCl (1 M, 25 μLmg−1). KOH (1 M, 28 μLmg−1) was added to the acidified solutions. The sample was centrifuged for 10 min causing a clear supernatant to form above a gel; the biphasic separation. The supernatant was extracted and dried by centrifugal evaporation and analysed by HPLC. All methods are outlined in Dickinson et al., 2019.