This dataset contains the results of 211 household surveys conducted in Mambwe District, Zambia, as part of a wider study looking at human and animal trypanosomiasis and changing settlement patterns in the area. The interviews were conducted from June 2013 to August 2013. The objective of the survey was to set the health of people and their animals in the context of overall household wellbeing, assets and access to resources. The topics covered included household demographics, human and animal health, access to and use of medical and veterinary services, livestock and dog demographics, livestock production, human and animal contacts with wildlife, crop and especially cotton production, migration, access to water and fuel use, household assets and poverty, resilience and values. The dataset has been anonymised by removing names of respondents, Global Positioning Satellite (GPS) location of their homes and names of interviewers. Household numbers were retained. Written consent was obtained prior to commencing all interviews. This research was part of a wider research project, the Dynamic Drivers of Disease in Africa Consortium (DDDAC), and these data contributed to the research carried out by the consortium. The research was funded by NERC with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at https://doi.org/10.5285/b1647138-49f5-4777-a39d-e7359bf7b98d