Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013)
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- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2017-03-24
- Dataset Reference Date ()
- 2016-11-21
- Identifier
- doi: / 10.5285/a55eea77-8401-49ba-921e-53e085dc8345
- Other citation details
- Simuunza, M., Anderson, N.E, Machila, N., Ndebe, J. , Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre 10.5285/a55eea77-8401-49ba-921e-53e085dc8345
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- © The University of Edinburgh
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- © University of Zambia
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- If you reuse this data, you should cite: Simuunza, M., Anderson, N.E, Machila, N., Ndebe, J. , Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345
- Spatial representation type
- textTable
- Topic category
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- Biota
- Health
- Begin date
- 2013-05-01
- End date
- 2013-11-30
- Code
- WGS 84
Distribution Information
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- dataset
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Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Tsetse surveys were conducted in May / June 2013 and November 2013. Tsetse flies were sampled using black screen fly rounds. Data were recorded on standard field sampling forms and then manually transferred to Microsoft Excel. Data were then checked for accuracy and incorrect spatial location coordinates removed. Tsetse flies were homogenized and DNA extracted. The resulting DNA was stored at minus 20 degrees Celsius until needed for Polymerase chain reaction (PCR). PCR analysis was performed using different primers to detect the different species of trypanosomes in the DNA. All PCR included a negative and a positive control. To determine the sources of blood meal (feeding patterns), tsetse DNA samples were subjected to PCR targeting the cytochrome oxidase b gene. The resulting PCR product was sequenced, and the sequences compared with those available in public databases by using nucleotide BLAST at NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to determine the animal species on which tsetse fed on. Data were collated in a Microsoft Excel spreadsheet and exported as a comma separated file for ingestion into the Environmental Information Data Centre (EIDC).
Metadata
- File identifier
- a55eea77-8401-49ba-921e-53e085dc8345 XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-03-01T11:28:03
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3