This dataset comprises conventional stomachs contents data for the biomass-dominant species of mesopelagic fish, particularly myctophid fish (Family Myctophidae) in the Scotia Sea. The data were collected by depth-discrete RMT25 net trawls deployed between 0-1000 m between the Antarctic Polar Front and the South Orkney Islands. Samples were collected repeatedly at nominal sampling stations during austral autumn 2004, spring 2006, summer 2008 and autumn 2009 for analyses examining the trophodynamics of Southern Ocean mesopelagic fish and their role in the food web. The data are the quantitative measures of diet composition (prey abundance, frequency and mass) observed from microscope analyses of individual fish stomachs per species.