This dataset contains aboveground net primary productivity (ANPP) estimates and litter layer depth measurements from five long-term grassland-to-woodland land use contrasts across England between 2018 and 2021. Each metric was measured once at each site. Litter layer depth was measured between November 2018 and March 2019 . Leaves were sampled for ANPP estimates in 2021. Each land use contrast was assigned a grassland and a woodland plot respectively. Each of these plots was further divided into three grids (grassland grids 1 to 3 / woodland grids 4 to 6) with grids 1 and 6 being the furthest apart. The contrast boundary is between grids 3 and 4. In each plot, nine sampling locations were randomly sampled (three in each grid). The five land use contrasts were: Gisburn (Gisburn-1, Gisburn-2), Alice Holt, Wytham Woods, and Kielder Forest. ANPP (g dry mass m-2 yr-1) was estimated from measured leaf dry matter content of the two dominant species in each grassland and woodland plot. Litter layer depth (cm) was measured with a ruler in the field. ANPP estimates and litter layer depth measurements in this dataset are co-located with soil physical, chemical and biological properties (0-1 m), soil hydraulic measurements (soil water release curves and hydraulic conductivity), and earthworm counts and identification. A file is provided to connect all co-located measurements. This project was part of the UK-SCAPE programme which started in 2018 and was funded by the Natural Environment Research Council as National Capability (award number NE/R016429/1). Full details about this dataset can be found at https://doi.org/10.5285/9156d263-4def-406d-bdc9-22a6821fe755