8dde3529-1cf7-4b0f-907d-a1631f38afd7 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:19:11 UK GEMINI 2.3 Genotype-phenotype association for mate choice in progeny of Drosophila simulans backcross 2022-04-13 publication https://catalogue.ceh.ac.uk/id/8dde3529-1cf7-4b0f-907d-a1631f38afd7 10.5285/8dde3529-1cf7-4b0f-907d-a1631f38afd7 doi: Erezyilmaz, D.F., Lu, K. (2022). Genotype-phenotype association for mate choice in progeny of Drosophila simulans backcross. NERC EDS Environmental Information Data Centre 10.5285/8dde3529-1cf7-4b0f-907d-a1631f38afd7 The data resource contains Drosophila simulans backcross female mate choice (phenotype) aligned with whole-genome genotypes of 784 female backcross progeny. The data were generated in a laboratory at Stony Brook University, New York and each test was conducted in 28.5 x 95mm plastic vials. Phenotype data was collected in 2015 and the final analysis was completed in 2021. Illumina libraries were sequenced at University of Oregon G3 Genomics Center, and the multiplexed shotgun genotyping (MSG) software was run at Janelia Research Campus, Virginia. The experiments were conducted to align the genotype of females with behavioural phenotype, female preference. Three test females were allowed to choose from three males of either species. ‘Choice’ was determined by copulation, and the species of ‘chosen’ male was recorded before gDNA was extracted from the female. Standard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing. Deniz Erezyilmaz was responsible for data collection and interpretation. Those genomes with incomplete or inconsistent genomes due to sparse reads/coverage were eliminated from the analysis. The research was funded through NERC grant NE/S010351/1. Investigating the dual role of mate choice genes in behavioural isolation and hybridization. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/8dde3529-1cf7-4b0f-907d-a1631f38afd7 Erezyilmaz, D. University of Oxford deniz.erezyilmaz@dpag.ox.ac.uk https://orcid.org/0000-0001-8416-8561 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Erezyilmaz, D.F. University of Oxford deniz.erezyilmaz@dpag.ox.ac.uk https://orcid.org/0000-0001-8416-8561 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Lu, K. State University of New York Downstate kenneth81891@yahoo.com author Erezyilmaz, D.F. University of Oxford deniz.erezyilmaz@dpag.ox.ac.uk owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian notPlanned Drosophila simulans Wikidata 2012-10-29 creation hybridisation GEMET - Concepts, version 4.1.3 2019-01-28 creation speciation assortative mating QTL analysis Assortative Hybridization Quantitative trait locus (QTL) analysis otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Erezyilmaz, D.F., Lu, K. (2022). Genotype-phenotype association for mate choice in progeny of Drosophila simulans backcross. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/8dde3529-1cf7-4b0f-907d-a1631f38afd7 English utf8 biota Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/8dde3529-1cf7-4b0f-907d-a1631f38afd7 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/8dde3529-1cf7-4b0f-907d-a1631f38afd7.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The Drosophila simulans backcross genotypes were obtained by Multiplexed Shotgun Genotyping (Andolfatto et al, 2011), and the files were thinned using David Stern’s (Janelia Research Campus) script, pull_thin (Github) were obtained by two-choice assay, females choosing between D. simulans and D. sechellia males. Standard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing., as described in Andolfatto, P., Davison, D., Erezyilmaz, D., Hu, T.T., Mast, J., Sunayama-Morita, T., & Stern, D.L. (2011). Multiplexed shotgun genotyping for rapid and efficient genetic mapping. In Genome Research (Vol. 21, Issue 4, pp. 610–617). Cold Spring Harbor Laboratory. https://doi.org/10.1101/gr.115402.110