Date (Publication)

2021-07-26

Date (Creation)

2021-06-24

Identifier

https://catalogue.ceh.ac.uk/id/8a8240a2-e8ed-495d-ae93-c35200956764

Identifier

doi: / 10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

Other citation details

Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre 10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

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Controlled

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This resource is available under the terms of the Open Government Licence

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If you reuse this data, you should cite: Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

Spatial representation type

textTable Text, table

Distance

1  urn:ogc:def:uom:EPSG::9001

Metadata language

EnglishEnglish

Character set

utf8 UTF8

Topic category

• Biota

Begin date

1995-01-01

End date

2014-12-31

Unique resource identifier

WGS 84

Distribution format

• Comma-separated values (CSV) ()

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dataset Dataset

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dataset

Statement

Blood samples (~25 μl) were collected and stored at room temperature in absolute ethanol. DNA was extracted using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions with modification of overnight lysis at 37 °C and a final DNA elution volume of 80 μl. DNA concentration and purity were quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific). The following thresholds were applied before samples were included for further analysis: (1) DNA concentration must be at least 15 ng/μl (based on a mean of three measurements), (2) the 260/280 absorbance ratio has to be between 1.8 and 2.0 for acceptable DNA purity, and (3) the 260/230 absorbance ratio must be higher than 1.8. DNA integrity was further validated by visualisation with ethidium bromide after electrophoresis on a 1.2% agarose gel, and all samples with evidence of DNA degradation were re-extracted or excluded. All DNA extractions that passed the above criteria were diluted to 3.3 ng/μl before telomere measurement. We used quantitative polymerase chain reaction to calculate a relative measure of telomere length that describes the amount of telomeric DNA in a sample relative to that of GAPDH, a constantly expressed reference gene.

File identifier

8a8240a2-e8ed-495d-ae93-c35200956764 XML

Metadata language

EnglishEnglish

Character set

ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1

Hierarchy level

dataset Dataset

Hierarchy level name

dataset

Date stamp

2025-11-13T16:12:50

Metadata standard name

UK GEMINI

Metadata standard version

2.3