Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014

These data consist of relative telomere length (RTL) measures from quantitative polymerase chain reaction, of Seychelles warbler birds on Cousin Island, Seychelles. The data were collected by the Seychelles Warbler Project in 1995-2014. Data include bird identity, sex, age, birth period, qPCR plate identity, RTL, technician, territory, field period, mum ID, dad ID, mum age at conception, dad age at conception, dominant female ID in the natal territory, dominant male ID in the natal territory Full details about this dataset can be found at https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

Identification info

Metadata Language: English (en)

Character set: utf8

Dataset Reference Date (): 2021-07-26

Dataset Reference Date (): 2021-06-24

Identifier: https://catalogue.ceh.ac.uk/id/8a8240a2-e8ed-495d-ae93-c35200956764

Identifier: doi: / 10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

Other citation details: Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre 10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

University of Leeds - Sparks, A.M.

University of East Anglia - Spurgin, L.G.

University of Groningen - van der Velde, M.

University of East Anglia - Fairfield, E.A.

University of Groningen - Komdeur, J.

University of Sheffield - Burke, T.

University of East Anglia - Richardson, D.S.

University of Groningen - Dugdale, H.L.

University of Groningen - Professor Hannah Dugdale

NERC EDS Environmental Information Data Centre

University of Leeds - Dr Hannah Dugdale

GEMET - INSPIRE themes, version 1.0 ()

Environmental Monitoring Facilities

Keywords

Life history
Telomere
Seychelles Warbler
Senescence
Biomarkers

Limitations on Public Access: otherRestrictions

Other constraints: Controlled

Use constraints: otherRestrictions

Other constraints: This resource is available under the terms of the Open Government Licence

Use constraints: otherRestrictions

Other constraints: If you reuse this data, you should cite: Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764

Spatial representation type: textTable

Distance: 1 urn:ogc:def:uom:EPSG::9001

Topic category

Biota

Begin date: 1995-01-01

End date: 2014-12-31

Code: WGS 84

Distribution Information

Data format

Comma-separated values (CSV) ()

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Resource Locator: Supporting information
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Quality Scope: dataset

Other: dataset

Report

Dataset Reference Date (): 2010-12-08

Statement: Blood samples (~25 μl) were collected and stored at room temperature in absolute ethanol. DNA was extracted using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions with modiﬁcation of overnight lysis at 37 °C and a ﬁnal DNA elution volume of 80 μl. DNA concentration and purity were quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific). The following thresholds were applied before samples were included for further analysis: (1) DNA concentration must be at least 15 ng/μl (based on a mean of three measurements), (2) the 260/280 absorbance ratio has to be between 1.8 and 2.0 for acceptable DNA purity, and (3) the 260/230 absorbance ratio must be higher than 1.8. DNA integrity was further validated by visualisation with ethidium bromide after electrophoresis on a 1.2% agarose gel, and all samples with evidence of DNA degradation were re-extracted or excluded. All DNA extractions that passed the above criteria were diluted to 3.3 ng/μl before telomere measurement. We used quantitative polymerase chain reaction to calculate a relative measure of telomere length that describes the amount of telomeric DNA in a sample relative to that of GAPDH, a constantly expressed reference gene.

Metadata

File identifier: 8a8240a2-e8ed-495d-ae93-c35200956764 XML

Metadata Language: English (en)

Character set: ISO/IEC 8859-1 (also known as Latin 1)

Resource type: dataset

Hierarchy level name: dataset

Metadata Date: 2023-02-09T11:09:24

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Environmental Information Data Centre

Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK

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