8976bd5c-e91f-4612-880c-7d15aca12809 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2021-03-22 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Biometric and reproductive measurements for the bivalve Astarte crenata and seastar Ctenodiscus crispatus from the Western Barents Sea, summer 2017 2021-03-22 creation 2021-03-22 revision 2021-03-22 publication 2021-03-22 released 1.0 https://doi.org/10.5285/8976bd5c-e91f-4612-880c-7d15aca12809 doi GB/NERC/BAS/PDC/01478 https://data.bas.ac.uk/ Please cite this item as: Reed, A., Godbold, J., Solan, M., & Grange, L. (2021). Biometric and reproductive measurements for the bivalve Astarte crenata and seastar Ctenodiscus crispatus from the Western Barents Sea, summer 2017 (Version 1.0) [Data set]. UK Polar Data Centre, Natural Environment Research Council, UK Research & Innovation. https://doi.org/10.5285/8976bd5c-e91f-4612-880c-7d15aca12809 Quantification of morphological and reproductive traits in Astarte crenata and Ctenodiscus crispatus (oocyte size/gonad index), used in the analyses by Reed et al. 2021 (Ecology and Evolution) from the Western Barents Sea during summer 2017 across a North - South Transect intersecting the polar front. No credit. completed Reed, Adam University of Southampton Researcher author Godbold, Jasmin University of Southampton Researcher author Solan, Martin University of Southampton Researcher author Grange, Laura Bangor University Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Ecological Dynamics > Life History EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher functional biogeography gametogenesis interannual variability life-history reproductive plasticity theme Western Barents Sea Arctic place Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/ otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 url url crossReference url url crossReference url url largerWorkCitation textTable eng utf8 biota 29.23142 30.50174 74.49999 81.28249 2017-07-22 2017-08-01 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain bytes 225280 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=8976bd5c-e91f-4612-880c-7d15aca12809 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Specimens of Astarte crenata and Ctenodiscus crispatus were collected in July 2017 (JR16006, RRS James Clark Ross; Hopkins 2018) using a 1.25 m Agassiz trawl (AGT) towed for 15 minutes at a ship speed of 1 knot, at three stations along the 30E meridian across the approximate location of the Polar Front (B13, 74° 49 N (South of the polar front); B14, 76° 50 N (approximate location of the polar front); B16, 80° 06 N (North of the polar front)). Four trawls were conducted at each station to ensure sufficient spatial replication. Ctenodiscus crispatus were found at all stations, whereas A. crenata were only found at stations B13 and B16. Fauna were sieved over a 1 cm mesh and retained and fixed in 10% phosphate buffered formalin (4% formaldehyde) prior to morphological and histological examination. Morphology: Astarte crenata. To assess population dynamics, each individual (n = 159) was measured using a digital calliper (± 0.01mm), to record maximum shell length, height, and width. Soft tissue was removed from the shell with a scalpel and weighed (± 0.01 g). Observation of the dissected bivalves and a preliminary histological analysis revealed that this population of A. crenata do not have discrete gonads, but have germinal tissues infiltrating the visceral mass, particularly within the digestive diverticulum. This means that gonad index cannot be reliably calculated. Hence, to ensure reproductive maturity had been reached, only specimens >20 mm shell length were used for reproductive analysis. Whole animal histology was necessary, and it was not possible to calculate a gonad index or measure of energy storage in the digestive diverticulum. Ctenodiscus crispatus. To assess population dynamics, reproductive, and digestive condition of each animal, we measured each specimen, and calculated gonad and pyloric caeca indices. We evacuated sediment within the body cavity through the mouth by applying pressure to the dorsal surface whilst rinsing with seawater. In total, 324 individuals were measured (± 0.01 mm) from the centre of the mouth to the tip of the longest arm, and from the centre of the mouth to the madreporite interradius, blotted dry and weighed (± 0.01 g). Dissection of the dorsal epithelium of 151 individual C. crispatus above 15 mm arm length revealed the pyloric caeca and gonads as discrete paired organs, which were subsequently removed from two interradial sections by dissection and used to determine the total gonad and pyloric caeca indices, i.e., the ratio of gonad or pyloric caeca mass to whole body wet weight, expressed as a percentage. Reproductive traits: Sections of A. crenata demonstrated dense areas of gametogenesis. To ensure near maximum cross-sectional diameter was quantified, unique oocytes were measured only when a nucleus was visible. For, C. crispatus, as the nucleus remained visible across multiple 6 µm sections, oocytes were only measured when the nucleolus was visible. For comparison of oocyte sizes between each female and station (A. crenata, n = 24 (B13, n = 12; B16, n = 12); C. crispatus, n = 24 (B13, n = 8; B14, n = 8; B16, n = 8), we calculated the Equivalent Circular Diameter (ECD) by measuring the area of 100 oocytes of each female (i.e., 1200 and 800 oocytes per station). Full descriptions and additional information provided in Reed et al. 2021 Ecology and Evolution in main manuscript and supplementary information. Data collection: Instrumentation: Digital vernier caliper Balance (+/-0.000 g) Rotary microtome Nikon D5000 digital SLR camera Olympus BHS (BH-2) stereomicroscope Data quality: Standard protocols were followed, and data entry double checked by an independent person. Biometric measurements (i.e., arm length, interradial length, shell length, shell height and tumidity (width)) to two decimal places. Wet weight to four decimal places. Oocyte Equivalent Circular Diameter to two decimal places. asNeeded completed