The data contains nitrogen (N) offtake, N emissions (ammonia and nitrous oxide), soil parameters (pH, EC, NH4+, NO3−), biomass and grain production from a winter wheat field experiment located at Bangor University (Henfaes Research Station in North Wales) and Rothamsted Research (North Wyke (NW) in Devon). Data were collected between April 2017 and August 2017. Measurements and soil and plant samples were taken from 45 plots in a randomized complete block design. Sixteen extra mini-plots at the prevailing wind (south westerly) edge of the experiment site were used at NW for NH3 volatilization measurements. The treatments were control (C, without N application), food based digestate (D), food based digestate + the nitrification inhibitor 3,4-dimethylpyrazole (DMPP; D+NI), acidified food based digestate (AD), acidified food based digestate + the nitrification inhibitor 3,4-dimethylpyrazole (DMPP; AD+NI). Four rates of ammonium nitrate (NH4NO3; 75, 150, 225 and 300 kg N ha−1) were applied to compare yields and fertilizer replacement rate of the digestate treatments (targeted at 190 kg N ha−1). Soil pH, soil electrical conductivity (EC), soil NH4+ and soil NO3−, NH3 volatilization, N2O emissions were measured periodically for C, D, D+NI, AD and AD+NI and nitrogen concentration in grain and straw, grain yield and plant biomass for all treatments. Nitrogen offtake, losses and fluxes were used to determine the N use efficiency (NUE) and fertilizer replacement rate of the digestate treatments. Measurements were undertaken by members of staff from Bangor University, School of Environment, Natural Resources & Geography and Rothamsted Research, Sustainable Agricultural Sciences – North Wyke. Data was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC - Ref BB/N013468/1 Full details about this dataset can be found at https://doi.org/10.5285/836dfb2e-bc6f-47d2-b924-8261be671391