7b721c07-bc38-4815-8669-4675867663d0 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:17:50 UK GEMINI 2.3 OSGB 1936 / British National Grid Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover 2017-06-13 publication 2015-06-30 creation https://catalogue.ceh.ac.uk/id/7b721c07-bc38-4815-8669-4675867663d0 10.5285/7b721c07-bc38-4815-8669-4675867663d0 doi: Evans, T.M., Heard, M.S., Vanbergen, A.J., Cavers, S., Ennos, R. (2017). Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover. NERC Environmental Information Data Centre 10.5285/7b721c07-bc38-4815-8669-4675867663d0 This dataset details the paternity of progeny from Eschscholzia californica plants introduced to habitats comprising different floral cover. Data was collected in June 2015 at the Hillesden estate, Buckinghamshire, UK. Plants were genotyped at seven microsatellite markers before being introduced across the study site to form experimental arrays. Experimental arrays comprised of three E.californica plants separated by 1m and arranged in a triangular formation. A total of sixteen arrays were introduced across four 100 hectare replicate blocks, each separated by >500m. At the centre of each block, four experimental arrays were placed at 50m intervals along a 150m transect laid symmetrically across the boundary between an established wildflower patch and bare, fallow ground or grazed grassland (two arrays within the florally rich habitat and two arrays within the florally poor habitat). Upon maturation approximately 10 seeds were collected from each plant and genotyped. Fragment analysis was conducted and alleles were scored on Genemarker. Seeds were then manually scored as outcrossed or selfed and paternity was determined using Cervus. The dataset was part of a larger experiment looking at the effect of floral resources on the pollination services to isolated plants. Full details about this dataset can be found at https://doi.org/10.5285/7b721c07-bc38-4815-8669-4675867663d0 Evans, T. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk pointOfContact Evans, T.M. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0002-0177-6631 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Heard, M.S. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Vanbergen, A.J. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0001-8320-5535 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Cavers, S. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-2139-9236 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Ennos, R. University of Edinburgh rennos@ed.ac.uk author NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner notPlanned Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Eschscholzia californica Wikidata 2012-10-29 creation Hillesden Buckinghamshire GeoNames 2006-01-01 creation parentage paternity allele wildflower fallow ground grazed grassland Californian poppy otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © UK Centre for Ecology & Hydrology otherRestrictions If you reuse this data, you should cite: Evans, T.M., Heard, M.S., Vanbergen, A.J., Cavers, S., Ennos, R. (2017). Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover. NERC Environmental Information Data Centre https://doi.org/10.5285/7b721c07-bc38-4815-8669-4675867663d0 textTable English utf8 environment 2015-06-01 2015-06-30 -1.03 -0.965 51.936 51.979 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/sd/7b721c07-bc38-4815-8669-4675867663d0.zip Supporting information Supporting information available to assist in re-use of this dataset information https://data-package.ceh.ac.uk/data/7b721c07-bc38-4815-8669-4675867663d0 Download the data Download a copy of this data download dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 All open flowers were removed from the E. californica plants prior to their placement in pre-marked locations across the landscape. One bud on each plant was covered in a fine muslin to represent a 'pollinator excluded' flower. Plants remained in the field for 16 days to ensure full anthesis of new flowers and to allow for multiple pollination events. After this period, all fruit were tagged to ensure that only fruit development arising from the period of the field experiment were included in analyses. Plants were then collected and stored under controlled glasshouse conditions until fruit maturation. Ten progeny per plant from each of the field exposed plants were genotyped using 50mg of fresh leaf material and following standardised protocols. The incidence of self-fertilisation in plants from each habitat was calculated manually by individually comparing each successfully amplified progeny against their maternal plant. If, at each of the seven loci, the progeny was a complete match for the maternal genotype, or was homozygote for one of the maternal plants alleles, it was scored as selfed. Alternatively, if any novel alleles were observed in the progeny that were not present in the maternal plant, the progeny was classified as outcrossed. Paternity was determined using Cervus 3.0.7, where each progeny sample was listed detailing alleles at the seven microsatellite loci, specifying the known maternal sample as well as the potential paternal samples.