This data resource consists of a nutrient concentration time series for the River Frome at East Stoke, Dorset, gathered between January 2004 and February 2006. Total phosphorus concentration data was gathered for the full duration of the project. Soluble reactive phosphorus, total oxidisable nitrogen and dissolved reactive silicon concentration was only gathered from Jan 2005 until Feb 2006. Sampling interval varied throughout the monitoring period, from a minimum of two samples to day, to eight per day during storm events (with an average of three samples per day throughout the duration of the monitoring period). Samples were taken from the main flow of the river using an automatic water sampler, and analysed using standard colorometric methods, at the CEH Dorset laboratories. Further details of the field and laboratory procedures are outlined in Bowes MJ, Smith JT, Neal C. The value of high resolution nutrient monitoring: a case study of the River Frome, Dorset, UK. Journal of Hydrology 2009; 378: 82-96. Full details about this dataset can be found at https://doi.org/10.5285/7af4c236-5148-4d3d-815c-b6451aee67a5