Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats
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Identification info
- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2016-04-25
- Dataset Reference Date ()
- 2013-08-31
- Identifier
- doi: / 10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f
- Other citation details
- Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre 10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f
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- notPlanned
- GEMET - INSPIRE themes, version 1.0 ()
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- Habitats and Biotopes
- Limitations on Public Access
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- Other constraints
- no limitations
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- © University of St Andrews
- Use constraints
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- Other constraints
- If you reuse this data, you should cite: Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f
- Spatial representation type
- textTable
- Topic category
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- Oceans
- Begin date
- 2013-01-01
- End date
- 2013-08-31
Distribution Information
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- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Sediment colloidal carbohydrate concentration was determined through the collection of contact cores. Contact cores use liquid nitrogen to freeze the top 2mm of the sediment surface, excess unfrozen sediment is removed and the sample core is stored below -80 degree centigrade. The sample is weighed to determine wet weight and freeze dried to remove all water. Colloidal carbohydrate concentration is determined using the Dubois Phenol-Sulphuric assay. A sediment sub-sample (50-500mg, depending on site) is taken from dried contact core sediment and known volume of distilled water is added. Sample is vortexed and centrifuged then a set volume of supernatant is removed. Concentrated sulphuric acid and 5 percent phenol are added to the supernatant sample ensuring the ratio of sample: phenol: acid is kept at 1:1:5 by volume. Allow 35mins for colour to develop. Decant the samples into cuvettes and read the absorbance at 486.5nm on Thermo Biomate 5 spectrophotometer. Use figures from regression analysis of glucose standards to calculate colloidal carbohydrate concentration expressed as glucose equivalents in micrograms per gram (µg g-1). Note: zeroes indicate colloidal carbohydrate concentration below detectable level. Twenty two 1 x 1 m quadrats were randomly allocated to each mudflat and saltmarsh site using R (R Development Core Team, 2014) to specify four different spatial scales (A = 1 quadrat only, B = 3 quadrats at 1 m to 10 m apart, C = 6 quadrats at 10 m to 100 m apart, D = 12 quadrats at 100 m to 1000 m or site maximum).
Metadata
- File identifier
- 6c027bf9-b344-4178-b8b3-d78cf39f017f XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-02-27T16:28:30
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3