Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa
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- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2024-05-08
- Dataset Reference Date ()
- 2024-04-10
- Identifier
- doi: / 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b
- Other citation details
- Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b
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- If you reuse this data, you should cite: Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b
- Topic category
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- Biota
Distribution Information
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- Quality Scope
- nonGeographicDataset
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- nonGeographicDataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Sixty (60) lines from the DGRP were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed individually to infection overnight. Each treatment was replicated 5-10 times (707 samples in total). The experiment was split among 5 experimental blocks with a variable number of replicates tested in each, as described in the data file. Following infection (see above) pathogen shedding was measured by placing each individual fly inside a sterile Eppendorf and then quantifying the number of viable P. aeruginosa colony-forming units defecated each infected fly for 24h following oral infection. CFU (colony forming unit) plating was carried out on Pseudomonas sp. selective media (PIM) to select for P. aeruginosa CFUs only. Each fly was then transferred to Drosophila Activity Monitor (DAM) and the total number of activity events were measured for each fly individually measured at 1 min resolution until death (2500-~20,000 minutes). Data generated in the DAM included the total number of activity bouts recoded for each minute for the duration of the experiment (2500-~20,000 minutes). The values reported in the resource are the total number of activity bouts recoded per fly until death or until the end of the experiment. Mortality was recorded as the last detected movement in DAM.
Metadata
- File identifier
- 65c7085a-7bcf-481a-884a-8e1c9cfc395b XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- nonGeographicDataset
- Hierarchy level name
- nonGeographicDataset
- Metadata Date
- 2024-07-04T07:43:15
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3