The deep sea benthic biodiversity dataset encompasses a wide range of benthic sampling and observational activities carried out by the Scottish Association for Marine Science (SAMS) since 1973. Data include analyses of samples obtained by fish trawls, benthic imagery and core sampling. The principle regions covered are the Arctic, North Atlantic & Rockall Trough and the Portuguese coast. Sampling has been carried out on numerous cruises, funded through various initiatives (ranging from NERC to commercially-funded ventures). A wide range of methods and equipment were used to obtain the information. These include Agassiz trawls, bed hop cameras, dredges, grabs, epibenthic sledges, corers and landers. These data help to build up a temporal and spatial record of regional biodiversity and consequently are a valuable tool to monitor the state of marine habitats.