500193f7-2653-4696-8224-276a734ed6ab English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:16:24 UK GEMINI 2.3 WGS 84 Rates of fluorescein diacetate hydrolysis, phenol oxidation and potential denitrification in sand and gravel sediments from an agriculturally-impacted stream 2019-01-10 publication https://catalogue.ceh.ac.uk/id/500193f7-2653-4696-8224-276a734ed6ab 10.5285/500193f7-2653-4696-8224-276a734ed6ab doi: Ullah, S., Glover, L., Percival, A. (2019). Rates of fluorescein diacetate hydrolysis, phenol oxidation and potential denitrification in sand and gravel sediments from an agriculturally-impacted stream. NERC Environmental Information Data Centre 10.5285/500193f7-2653-4696-8224-276a734ed6ab The dataset contains measurements of fluorescein, dopachrome and nitrous oxide production from incubation experiments of streambed sediments. The sediments were collected in five pseudo-replicates from 0 to 10cm depth using a 5cm-diameter AMS slide hammer and trowel at two locations within the Wood Brook, Staffordshire, in June 2015. Samples were collected from two reaches within the stream, a mid-stream sandy reach and a downstream gravel reach. The sediment was used in laboratory incubation experiments to determine rates of fluorescein diacetate (FDA) hydrolysis (total microbial activity), extracellular phenol oxidase activity (recalcitrant carbon uptake) and potential denitrification from sand versus gravel sediments. Also included is the organic matter content of the sediments. The work was funded by the Natural Environment Research Council. Full details about this dataset can be found at https://doi.org/10.5285/500193f7-2653-4696-8224-276a734ed6ab Ullah, S. University of Birmingham/University of Keele s.ullah@bham.ac.uk https://orcid.org/0000-0002-9153-8847 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Glover, L. University of Keele EMAIL NOT PROVIDED author Percival, A. University of Keele/Dublin Institute of Technology EMAIL NOT PROVIDED author Ullah, S. University of Birmingham s.ullah@bham.ac.uk https://orcid.org/0000-0002-9153-8847 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication denitrification agriculture gravel sand sediment nitrogen cycle carbon cycle GEMET - Concepts, version 4.1.3 2019-01-28 creation streambed substrate type microbial activity phenol oxidation fluorescein diacetate hydrolysis activity extracellular phenol oxidase activity otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © Natural Environment Research Council otherRestrictions If you reuse this data, you should cite: Ullah, S., Glover, L., Percival, A. (2019). Rates of fluorescein diacetate hydrolysis, phenol oxidation and potential denitrification in sand and gravel sediments from an agriculturally-impacted stream. NERC Environmental Information Data Centre https://doi.org/10.5285/500193f7-2653-4696-8224-276a734ed6ab textTable English utf8 inlandWaters 2015-06-01 2015-06-30 -2.314 -2.277 52.815 52.831 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/sd/500193f7-2653-4696-8224-276a734ed6ab.zip Supporting information Supporting information available to assist in re-use of this dataset information https://data-package.ceh.ac.uk/data/500193f7-2653-4696-8224-276a734ed6ab Download the data Download a copy of this data download dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Sediment samples were collected from the streambed in June 2015 using an AMS slide hammer with a 5 cm diameter and a trowel to collect sediment from between 0 and 10 cm depth. Sediment was collected from two sites and five pseudo-replicates were collected from each site, and subsequently homogenised and sieved (2 mm) within 36 hours of collection, then stored cold until the beginning of the experiments. Sediment was incubated in three separate incubation experiments to determine rates of FDA hydrolysis, extracellular phenol oxidase activity and potential denitrification. FDA hydrolysis was determined by mixing air-dried sediment with 1 M tris-hydroxymethyl-aminomethane (THAM) buffer and 0.5 ml of FDA substrate, closing the flask and incubating at 37 °C for 3 hours. 2 ml of acetone was then added to the sediment to prevent further reaction. The samples were filtered and fluorescein concentration was determined using a spectrophotometer (Varian Cary UV-Vis). The concentration of fluorescein in the control was subtracted from that in the sample, and then normalised by the mass of soil used for that sample, and the number of hours of the incubation. Extracellular phenol oxidase activity was determined by mixing dried sediment with deionised water, and then adding 10 mM dihydroxy-phenylalanine. The samples were then shaken at 25 °C for 30 minutes, before centrifuging to terminate the reaction. The solution was then filtered and dopachrome concentration was measured using a spectrophotometer (Varian Cary UV-Vis). The potential denitrification was determined by adding stock solution to field-moist sediment in dark bottles. The stock solution used was ultrapure water for control experiments, nitrate solution for nitrate-spiked experiments, glucose solution for carbon-spiked experiments and nitrate+glucose solution for nitrate-carbon-spiked solutions. Sample bottles were made anoxic, acetylene gas was added to prevent the conversion of N2O to N2, and samples were shaken during incubation at 22 °C. Gas samples were taken from the headspace at zero, three and six hours. N2O concentrations in the gas samples were determined on an Agilent 7890A gas chromatograph – micro electron detector. Concentrations were converted to production per mass of sediment per hour by normalising the concentration of fluorescein and dopachrome by the mass of sediment used and the length of the incubation period, and calculating the difference in N2O concentration between zero and six hours and normalising by the mass of sediment used and the length of the incubation period. Results from the experiments were entered into an Excel spreadsheet and exported as a comma separated value file (.csv) for ingestion into the EIDC.