These data were collected from a preliminary investigation on the interaction between turbulence and biofilms, using the particle image velocimetry (PIV) technique, which provides spatially- and temporally-resolved velocity vector fields in water for different flow configurations. Seventeen different experiments were conducted with different boundary conditions for each one. The biofilm was developed on a 30-cm-long section permeable bed, the biofilm-covered section was then placed in the water channel test section for flow experiments. Flow rate was regulated by a variable frequency drive controlling the pump speed. Data was recorded at four pump frequencies. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4fecb4cc-e751-4752-9687-09ef92626f63