4b27e5d5-2ec4-4b72-9939-ab66c2818612 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:19:54 UK GEMINI 2.3 Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms 2024-05-13 publication 2023-03-10 creation https://catalogue.ceh.ac.uk/id/4b27e5d5-2ec4-4b72-9939-ab66c2818612 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612 doi: Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612 This dataset contains information on the performance of soil eubacteria inoculated into soil microcosms and exposed to a series of experimental droughts (or control conditions) in an experimental evolution study. Isolates of Bacillus and Pseudomonas included in the experimental evolution study had been sampled from the roots of Festuca ovina in the control treatment at the Buxton Climate Change Impacts Lab (BCCIL). Variables measured included estimates of microbial population size (via qPCR), microcosm soil respiration in response to substrate addition (in a substrate utilisation profiling assay) and water loss from the soil in response to the experimental drought/control treatments. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612 Hahn, C.Z. Jazz Pharmaceuticals webbaholic@gmx.de https://orcid.org/0000-0002-8399-4682 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Airey, G.A. University of Liverpool G.A.Airey@liverpool.ac.uk author Crawford, J.A. Woodland Trust JohnCrawford@woodlandtrust.org.uk author Sayer, E.J. Lancaster University e.sayer@lancaster.ac.uk https://orcid.org/0000-0002-3322-4487 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Stevens, C.J. Lancaster University c.stevens@lancaster.ac.uk https://orcid.org/0000-0002-2390-1763 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Paterson, S. University of Liverpool stevep11@liverpool.ac.uk https://orcid.org/0000-0002-1307-2981 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Whitlock, R. University of Liverpool r.whitlock@liverpool.ac.uk https://orcid.org/0000-0002-7067-8365 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Whitlock, R. University of Liverpool r.whitlock@liverpool.ac.uk https://orcid.org/0000-0002-7067-8365 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Liverpool EMAIL NOT PROVIDED owner Bacillus Pseudomonas Wikidata 2012-10-29 creation climate change drought grassland GEMET - Concepts, version 4.1.3 2019-01-28 creation soil bacteria experimental evolution soil ecology otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612 English utf8 biota environment Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/4b27e5d5-2ec4-4b72-9939-ab66c2818612 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/4b27e5d5-2ec4-4b72-9939-ab66c2818612.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Bacterial isolates from BCCIL (twelve from each of genera Bacillus and Pseudomonas) were raised in liquid media and used to inoculate sterilised soil in kilner jars (approx 100 ml soil in 500 ml jars). Each isolate of each bacterial taxon was inoculated into five replicate microcosms per moisture treatment (drought and control) and microcosms were assigned random positions within four experimental blocks. Microcosms in the control treatment were topped up weekly to a volumetric water content of 40%. Those in the drought treatment were subjected to a series of four simulated droughts lasting six weeks, where watering was not carried out. Soil moisture content determination and watering was carried out by assessing the mass of each kilner jar and the dried soil it initially contained, allowing controlled watering to the target soil water content. Substrate utilisation profiling was carried out by taking soil samples from the microcosms at the end of the final drought cycle, which were amended with different substrates (water, glucose, cellulose, artificial root exudate)in MicroResp(TM) colourimetric microplates, before CO2 efflux detection on a FLUOstar Omega spectrophotometer (BMG Labtech, Ortenberg, Germany). q-PCR was carried out by taking soil samples from the microcosms in the experimental evolution. RNA was extracted from the soil samples and amplified using qPCR primers specific to the isolates which had been used to inoculate the microcosms.