475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:25:58 UK GEMINI 2.3 Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA 2025-02-12 publication https://catalogue.ceh.ac.uk/id/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e doi: Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e Connolly, J.A. University of Manchester jack.connolly@manchester.ac.uk https://orcid.org/0000-0003-3643-5578 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Del Carratore, F. University of Manchester francesco.del-carratore@liverpool.ac.uk https://orcid.org/0000-0003-1647-7818 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Schmidt, K. University of Manchester kamila.schmidt@manchester.ac.uk author Bisesi, A. University of Minnesota bises004@umn.edu author Martinson, J.N.V. University of Minnesota jonmartinson1@gmail.com author Chua, J. University of Manchester jeremy.chua@manchester.ac.uk author Kuhs, M. University of Minnesota kuhsx003@umn.edu author Boneza, M. University of Minnesota bonez001@umn.edu author Heinsch, S. University of Minnesota heins049@umn.edu author Kinkel, L. University of Minnesota kinkel@umn.edu https://orcid.org/0000-0002-5331-5589 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Smanski, M. University of Minnesota smanski@umn.edu author Harcombe, W.R. University of Minnesota harcombe@umn.edu author Breitling, R. University of Manchester rainer.breitling@manchester.ac.uk https://orcid.org/0000-0001-7173-0922 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Takano, E. University of Manchester eriko.takano@manchester.ac.uk https://orcid.org/0000-0002-6791-3256 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author University of Manchester jack.connolly@manchester.ac.uk owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Connolly, J.A. University of Manchester jack.connolly@manchester.ac.uk pointOfContact Species Distribution theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Biodiversity bacteria microbiology co-culture microbiome streptomyces mass spectrometry actinomycetes metabolomics untargeted mzml otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e English utf8 biota .mzML NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://catalogue.ceh.ac.uk/datastore/eidchub/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e Download the data Download a copy of this data fileAccess https://data-package.ceh.ac.uk/sd/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.