The Environmental Change Network (ECN) coarse-grain and woodland protocols were used to assess the vegetation within the deer exclosure plots at the ECN Wytham site. In the ECN coarse-grain protocol, 2m x 2m plots are randomly selected on the site. Species presence is recorded in each of the 25 40cm x 40cm cells within the plots. In the ECN woodland protocol seedlings, diameter at breast height (dbh), tree height and dominance are recorded in 10m x 10m plots. ECN is the UK's long-term environmental monitoring programme. It is a multi-agency programme sponsored by a consortium of fourteen government departments and agencies. These organisations contribute to the programme through funding either site monitoring and/or network co-ordination activities. These organisations are: Agri-Food and Biosciences Institute, Biotechnology and Biological Sciences Research Council, Cyfoeth Naturiol Cymru - Natural Resources Wales, Defence Science & Technology Laboratory, Department for Environment, Food and Rural Affairs, Environment Agency, Forestry Commission, Llywodraeth Cymru - Welsh Government, Natural England, Natural Environment Research Council, Northern Ireland Environment Agency, Scottish Environment Protection Agency, Scottish Government and Scottish Natural Heritage. Full details about this dataset can be found at https://doi.org/10.5285/3ff83bf2-4864-4a7c-9ec0-df1811a99a71