3ca94e73-57dc-4fcd-afe2-82a4e491970a English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:26:59 UK GEMINI 2.3 OSGB 1936 / British National Grid River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB 2025-03-27 publication https://catalogue.ceh.ac.uk/id/3ca94e73-57dc-4fcd-afe2-82a4e491970a 10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a doi: Mackay, E.B., Feuchtmayr, H., Thackeray, S.J., Callaghan, N., Marshall, M., Rhodes, G., Yates, C., Johnes, P.J., Maberly, S.C. (2025). River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB. NERC EDS Environmental Information Data Centre 10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a The data are from bioassay experiments from 25 river sites across Great Britain to investigate the growth response of river phytoplankton to different inorganic and organic nutrient additions and compare that to in situ nutrient concentrations. The data includes calculated growth responses to inorganic and organic nutrient additions, based on experimental incubations and background nutrient data collected from sites at the same time as water for the experimental incubations. Full details about this dataset can be found at https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a Mackay, E.B. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0001-5697-7062 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Feuchtmayr, H. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Thackeray, S.J. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Callaghan, N. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Marshall, M. Bangor University m.marshall@bangor.ac.uk author Rhodes, G. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Yates, C. Atkins Realis Christopher.Yates@atkinsrealis.com author Johnes, P.J. Bristol University penny.johnes@bristol.ac.uk author Maberly, S.C. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Mackay, E.B. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner notPlanned Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication nutrient phytoplankton GEMET - Concepts, version 4.1.3 2019-01-28 creation Water quality Great Britain Bioassay Organic nutrients River phytoplankton Nutrient limitation DOMAINE otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Mackay, E.B., Feuchtmayr, H., Thackeray, S.J., Callaghan, N., Marshall, M., Rhodes, G., Yates, C., Johnes, P.J., Maberly, S.C. (2025). River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a textTable 100 English utf8 environment 2017-09-05 2017-10-26 -8.648 1.768 49.864 60.861 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/3ca94e73-57dc-4fcd-afe2-82a4e491970a Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/3ca94e73-57dc-4fcd-afe2-82a4e491970a.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Raw water samples were collected at each site on a single sampling occasion and kept at 4°C in the dark and transported to the laboratory within 48 hours. They were acclimatised in a constant temperature room at 20°C. Water was filtered through a 100 µm mesh to remove any zooplankton or large detritus present, 35 mL of sample was transferred to 50 mL boiling tubes. 12 different nutrient treatments were applied at approximately Redfield ratio [N: 90 µmol L-1 (1.261 mg N L-1) and P: 6 µmol L-1 (0.186 mg P L-1)] in triplicate to quantify growth responses to both organic N and P. These included: 1) a control with no addition of nutrients, 2) inorganic N only, 3) inorganic P only and 4) inorganic N and P, 5-8) four organic N additions and 9- 12) four organic P additions. Tubes were stoppered with foam bungs enabling gas exchange and incubated for 14 days at 20°C under a photon irradiance of 80 – 120 µmol m-2 s-1 (photosynthetically available radiation, Macam Q102) and an 18 h light, 6 h dark cycle. After the incubation period, tube contents including biofilm were resuspended by scraping and vortex mixing before being filtered onto a Whatman GF/C glass fibre filter (nominal pore size 1.2 µm) and frozen. Filters were later defrosted, and chlorophyll a extracted in hot methanol and measured spectrophotometrically. Growth response was determined as a change in the concentration of chlorophyll a in the treatment relative to the relevant control calculated as a natural log response ratio. For the water chemistry data (measured in mg/L), a Skalar++ multi-channel continuous flow autoanalyzer (Skalar Analytical B.V., The Netherlands) was used to carry out inorganic nutrient analysis, including total and total dissolved N and P (TN or TP, TDN or TDP) following persulphate digestion of unfiltered and filtered (pre-leached 0.45 µm cellulose nitrate) samples respectively, and dissolved inorganic N (DIN), including total ammoniacal nitrogen (NH3 and NH4), or soluble reactive P (SRP) on filtered samples. Dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP), were determined by difference between TDN and DIN or TDP and SRP, respectively. Data from these analyses were checked and entered into spreadsheets for later analysis.