Hourly temperature, environmental parameter and Daphnia magna phenotypic and genetic change in mesocosms with manipulated experimental parameters, 2017-2019
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- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2022-06-10
- Identifier
- doi: / 10.5285/2ae5e8d3-be36-4517-b80c-c6b91792b769
- Other citation details
- Brunner, F.S. , Reynolds, A., Price, S., White, N., Sadler, D.E., Wilson, I.W., Atkinson, D.A., Paterson, S., Plaistow, S.J. (2022). Hourly temperature, environmental parameter and Daphnia magna phenotypic and genetic change in mesocosms with manipulated experimental parameters, 2017-2019. NERC EDS Environmental Information Data Centre 10.5285/2ae5e8d3-be36-4517-b80c-c6b91792b769
- GEMET - INSPIRE themes, version 1.0 ()
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- Environmental Monitoring Facilities
- Wikidata
- GEMET - Concepts, version 4.1.3
- Keywords
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- Environmental risk
- Phenotypic plasticity
- clonal diversity
- Experimental evolution
- Fitness traits
- Mesocosms
- Freshwater ecology
- Heatwaves
- Limitations on Public Access
- otherRestrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions
- Use constraints
- otherRestrictions
- Other constraints
- If you reuse this data, you should cite: Brunner, F.S. , Reynolds, A., Price, S., White, N., Sadler, D.E., Wilson, I.W., Atkinson, D.A., Paterson, S., Plaistow, S.J. (2022). Hourly temperature, environmental parameter and Daphnia magna phenotypic and genetic change in mesocosms with manipulated experimental parameters, 2017-2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/2ae5e8d3-be36-4517-b80c-c6b91792b769
- Spatial representation type
- textTable
- Topic category
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- Biota
))
- Begin date
- 2017-01-01
- End date
- 2019-12-31
Distribution Information
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Comma-separated values (CSV)
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Supporting information
Supporting information available to assist in re-use of this dataset
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Supporting information
Supporting information available to assist in re-use of this dataset
- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Daphnia magna clones used in the experiment were isolated from Brown Moss in Shropshire (52°57'01"N 2°39'05"W) and Lake Ring in Denmark (55°57′51.83″N, 9°35′46.87″E) as hatched resting eggs. How plastic each clone is was assayed by rearing clones in common garden studies using four different environments. Forty-two mesocosms with different numbers of plastic and non-plastic Daphnia magna clones were established at Ness botanic gardens (53°16'20"N 3°02'34"W). Heatwave induction and hourly temperature data were managed using heating elements and temperature sensors at 50cm depth connected to Campbell Scientific CR6 Wi-Fi data loggers connected to a virtual server. Environmental parameter data were collected using hand-held meters every 2-weeks. Water chemistry data were collected every 3-months between 2017-2019 and analysed using a spectrophotometer (see supporting doc for details). The life-history and thermal tolerance of D. magna in mesocosms was assayed 5 times a year before and after heatwaves (September 2017 March 2018, June 2018, August 2018, and October 2018) by sampling animals from each mesocosm and rearing an offspring in common garden study. Clone-frequency changes were monitored in a subset of mesocosms at 8 timepoints between 2017-2019 using four microsatellite markers. Community dynamics in each mesocosm were assessed at 8-timepoints between 2017-2019 using depth integrated samples from four locations across a mesocosm for a total of 10L of water, which was then filtered through 100 micromitre sieves and stored in 15% glycerol at 20 oC prior to counting. All data were checked, verified and processed for analysis.
Metadata
- File identifier
- 2ae5e8d3-be36-4517-b80c-c6b91792b769 XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2023-11-09T19:12:08
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3