Date (Publication)

2014-05-06

Identifier

CEH:EIDC: / 1395928628829

Identifier

https://catalogue.ceh.ac.uk/id/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

Identifier

doi: / 10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

Other citation details

Griffiths, R., Pywell, R.F., Peyton, J., Nuttall, P., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments. NERC Environmental Information Data Centre 10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

Maintenance and update frequency

unknown Unknown

Maintenance note

not planned

GEMET - INSPIRE themes, version 1.0

• Environmental Monitoring Facilities

Access constraints

otherRestrictions Other restrictions

Other constraints

no limitations

Use constraints

otherRestrictions Other restrictions

Other constraints

This resource is made available under the terms of the Open Government Licence

Use constraints

otherRestrictions Other restrictions

Other constraints

© UK Centre for Ecology & Hydrology

Use constraints

otherRestrictions Other restrictions

Other constraints

If you reuse this data, you should cite: Griffiths, R., Pywell, R.F., Peyton, J., Nuttall, P., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments. NERC Environmental Information Data Centre https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b

Spatial representation type

textTable Text, table

Metadata language

EnglishEnglish

Character set

utf8 UTF8

Topic category

• Biota
• Environment
• Inland waters
• Location

Begin date

2013-10-01

End date

2014-03-20

Unique resource identifier

OSGB 1936 / British National Grid

Distribution format

• Comma-separated values (CSV) ()

OnLine resource

Supporting Information

Supporting information available to assist in re-use of this dataset

OnLine resource

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Hierarchy level

dataset Dataset

Other

dataset

Statement

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

File identifier

296ded8e-2c80-4a01-98cc-e71e3fa3fa1b XML

Metadata language

EnglishEnglish

Character set

ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1

Hierarchy level

dataset Dataset

Hierarchy level name

dataset

Date stamp

2024-03-01T12:23:21

Metadata standard name

UK GEMINI

Metadata standard version

2.3