The dataset contains abundance data of airborne pollen (including Anthoxanthum odoratum (sweet vernal-grass), Arrhenatherum elatius (false oat-grass), Cynosurus cristatus (crested dog's-tail), Dactylis glomerata (cock's-foot), Lolium perenne (perennial ryegrass), Phleum pratense (Timothy), Poa pratensis (smooth meadow-grass), grass species within the genera Alopecurus/Agrostis, and one probe that was found to be degenerate and unable to discriminate grass species. Here we used qPCR to track the seasonal progression of airborne grass pollen, in time and space. To do this we collected aerial samples from thirteen sites across the UK during the pollen seasons (May to September) of 2016 and 2017. Full details about this dataset can be found at https://doi.org/10.5285/28208be4-0163-45e6-912c-2db205126925