NERC Data Catalogue Service
Abundance of airborne pollen for nine grass species, measured by qPCR, UK, 2016-2017
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Identification info
- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2020-12-15
- Identifier
- doi: / 10.5285/28208be4-0163-45e6-912c-2db205126925
- Other citation details
- Brennan, G., Creer, S., Griffith, G. (2020). Abundance of airborne pollen for nine grass species, measured by qPCR, UK, 2016-2017. NERC Environmental Information Data Centre 10.5285/28208be4-0163-45e6-912c-2db205126925
- GEMET - INSPIRE themes, version 1.0 ()
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- Environmental Monitoring Facilities
- Limitations on Public Access
- otherRestrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions
- Use constraints
- otherRestrictions
- Other constraints
- If you reuse this data, you should cite: Brennan, G., Creer, S., Griffith, G. (2020). Abundance of airborne pollen for nine grass species, measured by qPCR, UK, 2016-2017. NERC Environmental Information Data Centre https://doi.org/10.5285/28208be4-0163-45e6-912c-2db205126925
- Spatial representation type
- textTable
- Distance
- 1 urn:ogc:def:uom:EPSG::9001
- Topic category
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- Biota
))
- Begin date
- 2016-05-01
- End date
- 2017-09-30
Distribution Information
- Data format
-
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Comma-separated values (CSV)
()
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Comma-separated values (CSV)
()
- Resource Locator
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Supporting information
Supporting information available to assist in re-use of this dataset
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Supporting information
Supporting information available to assist in re-use of this dataset
- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- The qPCR data was collected using a QuantStudio 6 Flex Real-Time qPCR machine (ThermoFisher Scientific). Species-specific primers were designed to target the ITS2 region of abundant grass species in the UK including Anthoxanthum odoratum (sweet vernal-grass), Arrhenatherum elatius (false oat-grass), Cynosurus cristatus (crested dog's-tail), Dactylis glomerata (cock's-foot), Lolium perenne (perennial ryegrass), Phleum pratense (Timothy), Poa pratensis (smooth meadow-grass), grass species within the genera Alopecurus/Agrostis, and one probe that was found to be degenerate and unable to discriminate grass species. Of 1,400 daily aerial samples, 1,210 were selected for downstream molecular analysis. Samples were excluded if pollen could not be reliably extracted due to large volumes of rainwater in collection tubes. Quantitative PCR runs with PCR efficiencies less than 85% and greater than 115% were not used for further analysis (efficiency of qPCR data used in downstream analysis ranged between 88.5% and 106%). Data points with a large standard deviation between three technical replicates (>6.95, based on the upper quartile range of the data) were removed. In addition, samples which amplified before 10 cycles and after 38 cycles were removed to reduce the chance of detecting false positive or false negative amplification respectively. The reliability of the data was evaluated based on the positive and negative controls.
Metadata
- File identifier
- 28208be4-0163-45e6-912c-2db205126925 XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-03-12T10:48:14
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
