27e7df36-d689-4803-93b4-c15935693b83 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:15:02 UK GEMINI 2.3 OSGB 1936 / British National Grid Common juniper single nucleotide polymorphism (SNP) and microsatellite (SSR) genotype data from individuals sampled from 18 natural UK populations in 2019 2024-11-11 publication 2024-10-17 creation https://catalogue.ceh.ac.uk/id/27e7df36-d689-4803-93b4-c15935693b83 10.5285/27e7df36-d689-4803-93b4-c15935693b83 doi: Baker, J., A’Hara, S., Perry, A., Cottrell, J., Ennos, R., Green, S., Cavers, S. (2024). Common juniper single nucleotide polymorphism (SNP) and microsatellite (SSR) genotype data from individuals sampled from 18 natural UK populations in 2019. NERC EDS Environmental Information Data Centre 10.5285/27e7df36-d689-4803-93b4-c15935693b83 This dataset contains genotype data of Single Nucleotide Polymorphisms (SNPs) and Simple Sequence Repeats (SSRs) from 18 populations of UK juniper, including the two subspecies hemisphaerica and nana. Plant material was collected in 2019. These data were gathered as part of a project on juniper conservation genetics that also includes an analysis of the quantitative genetics of UK junipers. The main goal of the project is to evaluate the standing genetic variation of populations using both quantitative and population genetic analyses. Full details about this dataset can be found at https://doi.org/10.5285/27e7df36-d689-4803-93b4-c15935693b83 Baker, J. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0009-0003-0514-4644 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Baker, J. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0009-0003-0514-4644 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact A’Hara, S. Forest Research stuart.ahara@forestresearch.gov.uk https://orcid.org/0000-0001-7891-4513 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Perry, A. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0002-7889-7597 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Cottrell, J. Forest Research joan.cottrell@forestresearch.gov.uk https://orcid.org/0000-0001-6355-1326 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Ennos, R. University of Edinburgh rennos@ed.ac.uk https://orcid.org/0000-0001-5401-297X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Green, S. Forest Research sarah.green@forestresearch.gov.uk author Cavers, S. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-2139-9236 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Juniperus communis Wikidata 2012-10-29 creation Biodiversity population genetics Britain juniper genetic data SNP SSR neutral genetic variation otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Baker, J., A’Hara, S., Perry, A., Cottrell, J., Ennos, R., Green, S., Cavers, S. (2024). Common juniper single nucleotide polymorphism (SNP) and microsatellite (SSR) genotype data from individuals sampled from 18 natural UK populations in 2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/27e7df36-d689-4803-93b4-c15935693b83 textTable 10 English utf8 biota 2019-01-01 2019-12-31 -8.648 1.768 49.864 60.861 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/27e7df36-d689-4803-93b4-c15935693b83 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/27e7df36-d689-4803-93b4-c15935693b83.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 For the SNP genotyping plant material was collected in 2019. Needles were freeze-dried and finely chopped using a razor blade, and then using a Retsch MM 300 mixer mill at a frequency of 30/s for a total of 2 minutes with metal beads in each Eppendorff tube to grind samples. Razor blades, scissors and lab surfaces were cleaned with 2% ethanol between processing samples. DNA was extracted for each sample using a Qiagen DNeasy Plant Pro kit following the manufacturer’s instructions. SNPs were identified and sequenced for this study by the Plant Genomic Resources Centre (CNRGV, INRAE, France; https://cnrgv.toulouse.inrae.fr/). A sub-sample of these were used to develop two Sequenom SNP chips which provided data on 74 SNP loci in the samples analysed in this study For the SSR genotyping a new set of microsatellite markers was developed for this study. Juniper DNA samples were sent to Microsynth Ecogenics (https://www.ecogenics.ch/home.html) to identify nuclear microsatellite (SSR) polymorphisms. Of the 285 identified microsatellites 48 were tested using multiplex PCR and gel electrophoresis which returned 6 polymorphic markers which were consistently amplified by PCR. PCR products were run on a Licor 4300 DNA sequencer and the allele sizes scored by eye using a size marker as reference.