This dataset consists of macrophyte species records, sampled from headwater streams during a survey in 2007. Stream macrophytes in Countryside Survey are surveyed using the standard MTR (Mean Trophic Rank) protocol, which records the presence and extent (on a categorical scale) of macrophytes in a 100m reach. Data were collected under the Countryside Survey long term monitoring project managed by the Centre for Ecology & Hydrology. The Countryside Survey is a unique study or 'audit' of the natural resources of the UK's countryside. The sample sites are chosen from a stratified random sample, based on a 15 by 15 km grid of GB. Headwater stream surveys have been carried out in 1990, 1998 and 2007 with repeated visits to the majority of sites. The countryside is sampled and surveyed using rigorous scientific methods, allowing us to compare new results with those from previous surveys. In this way we can detect the gradual and subtle changes that occur in the UK's countryside over time. In addition to headwater stream data, soil data, habitat areas, vegetation species data and linear habitat data are also gathered by Countryside Survey. Full details about this dataset can be found at https://doi.org/10.5285/249a90ec-238b-4038-a706-6633c3690d20