Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016)
The data set includes the results of a laboratory analysis in 2016, investigating the presence of trypanosomes and prevalence of tsetse endosymbionts in tsetse flies. The tsetse flies were sampled in Hurungwe District, Mashonaland West Province, Zimbabwe, from February 2014 to November 2014. Flies were sampled using a combination of Epsilon traps and fly rounds, both established techniques for sampling tsetse. Tsetse were stored prior to laboratory analysis using Polymerase chain reaction (PCR) techniques in 2016. The data include two species of tsetse, Glossina pallidipes and Glossina morsitans morsitans. Trypanosome species investigated include Trypanosoma brucei s.l., T. b. rhodesiense, T. vivax, T. congolense, T. simiae, T.simiae (Tsavo) and T. godfreyi. Endosymbionts included in the study were Sodalis glossinidius and Wolbachia spp. Hurungwe District is the only sleeping sickness focus in Zimbabwe and an increase in cases had been detected in years preceding this study. The objective of the study was to investigate the trypanosome species present in the tsetse population and their association with tsetse endosymbionts. This study was conducted as part of research into the relationship between trypanosomiasis, well-being and ecosystems by the Dynamic Drivers of Disease in Africa Consortium (DDDAC). The research was funded by NERC with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at
https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
Simple
- Date (Publication)
- 2017-04-21
- Date (Creation)
- 2016-04-19
- Identifier
- doi: / 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Other citation details
- Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
Point of contact
The Royal (Dick) School of Veterinary Studies, University of Edinburgh
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Neil Anderson
Author
Department of Livestock and Veterinary Services, Zimbabwe
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Shereni, W.
https://orcid.org/0000-0002-9427-4349
Author
Department of Livestock and Veterinary Services, Zimbabwe
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Nyakupinda, L.
https://orcid.org/0000-0003-4768-0392
Author
Central Veterinary Research and Diagnostic Laboratory, Zimbabwe
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Gwenhure, L.F.
https://orcid.org/0000-0001-8299-3082
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- © The University of Edinburgh
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- © Central Veterinary Research and Diagnostic Laboratory, Zimbabwe
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- © Department of Livestock and Veterinary Services, Zimbabwe
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Spatial representation type
- textTable Text, table
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
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- Biota
- Health
- Begin date
- 2014-02-01
- End date
- 2016-04-12
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E
W
- Unique resource identifier
- WGS 84
- Distribution format
-
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Comma-separated values (CSV)
()
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Comma-separated values (CSV)
()
- OnLine resource
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Supporting information
Supporting information available to assist in re-use of this dataset
- Hierarchy level
- dataset Dataset
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- dataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- The data presents the molecular analysis of 101 Glossina morsitans morsitans and 108 Glossina pallidipes tsetse sampled in Hurungwe District, Mashonaland West Province, Zimbabwe. Tsetse were sampled using Epsilon traps and fly rounds as described by Shereni et al. (2016). Tsetse were stored in cryotubes with desiccant beads at room temperature. DNA was extracted from the tsetse using Qiagen DNeasy kits and then subjected to polymerase chain reactions (PCR) to detect the primary endosymbiont, Wigglesworthia glossindia. Samples from which no DNA was amplified were discounted from further molecular analysis. All remaining samples were subjected to PCR reactions to detect African trypanosomes and the secondary tsetse symbionts, Sodalis glossinidius and Wolbachia. All PCRs included a negative and a positive control. The data were collated in an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.
- File identifier
- 1e435dd3-ed93-4961-a136-ffa477731e1c XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- dataset Dataset
- Hierarchy level name
- dataset
- Date stamp
- 2024-03-01T11:26:07
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
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