NERC Data Catalogue Service
Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm
In this mesocosm study, three types of wet wipes (plastic containing, and home and commercially compostable) colonised with E. coli were buried in beach sand and their degradation, tensile strength, and concentration of E. coli was quantified over 15 weeks. Wipes were firstly passed through a series of treatments to simulate their journey from the bathroom to the beach. Water characteristics (pH, salinity) of these water types were measured. Full details about this nonGeographicDataset can be found at
https://doi.org/10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
Simple
- Date (Publication)
- 2024-07-15
- Identifier
- doi: / 10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
- Other citation details
- Metcalf, R., Fellows, R., White, H.L., Quilliam, R. (2024). Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm. NERC EDS Environmental Information Data Centre 10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
- GEMET - INSPIRE themes, version 1.0
-
- Human Health and Safety
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Metcalf, R., Fellows, R., White, H.L., Quilliam, R. (2024). Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
-
- Health
- Environment
- Distribution format
-
-
Comma-separated values (CSV)
()
-
Comma-separated values (CSV)
()
- OnLine resource
-
Download the data
Download a copy of this data
- OnLine resource
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Supporting information
Supporting information available to assist in re-use of this dataset
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- nonGeographicDataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- At weekly timepoints (for 15 weeks), four of each wet wipe type were randomly selected, and removed from the mesocosm using sterile forceps. The loosely adhering sand was shaken off and each wet wipe transferred into a sterile glass vial containing 20 ml phosphate-buffered saline (PBS). Vials were then vortexed at 1500 rpm for 5 min and E. coli enumerated by filtering the PBS through 0.45 μm filters, which were placed onto selective media and incubated at 37°C. The wet wipes were removed from the glass vials and left to dry overnight. The following day, a 50 N digital force meter was used to quantify tensile strength by measuring the force required to break each wipe. Wipes were also photographed to determine degradation visually. Background bacterial concentrations were measured by filtering 100 ml water through 0.45 μm cellulose acetate membranes and placed onto selective media.
- File identifier
- 19a8083b-afa4-40b9-91bf-7a529eea870d XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- nonGeographicDataset
- Date stamp
- 2025-03-21T09:38:38
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
