Date (Publication)

2017-04-21

Date (Creation)

2010-01-01

Identifier

https://catalogue.ceh.ac.uk/id/10c6df00-c7ef-444b-951f-33a2c0072bec

Identifier

doi: / 10.5285/10c6df00-c7ef-444b-951f-33a2c0072bec

Other citation details

Hewitt, D. (2017). Influence of Jasmonic acid on the response of pasture to ozone. NERC Environmental Information Data Centre 10.5285/10c6df00-c7ef-444b-951f-33a2c0072bec

Maintenance and update frequency

notPlanned Not planned

GEMET - INSPIRE themes, version 1.0

• Environmental Monitoring Facilities

Wikidata

• Trifolium repens
• Jasmonic acid

GeoNames

• Abergwyngregyn

Keywords

• Environmental risk
• Pollution

Access constraints

otherRestrictions Other restrictions

Other constraints

no limitations

Use constraints

otherRestrictions Other restrictions

Other constraints

This resource is made available under the terms of the Open Government Licence

Use constraints

otherRestrictions Other restrictions

Other constraints

© UK Centre for Ecology & Hydrology

Use constraints

otherRestrictions Other restrictions

Other constraints

If you reuse this data, you should cite: Hewitt, D. (2017). Influence of Jasmonic acid on the response of pasture to ozone. NERC Environmental Information Data Centre https://doi.org/10.5285/10c6df00-c7ef-444b-951f-33a2c0072bec

Spatial representation type

textTable Text, table

Metadata language

EnglishEnglish

Character set

utf8 UTF8

Topic category

• Environment

Begin date

2015-05-28

End date

2015-06-24

Unique resource identifier

WGS 84

Distribution format

• Comma-separated values (CSV) ()

OnLine resource

Download the data

Download a copy of this data

OnLine resource

Supporting information

Supporting information available to assist in re-use of this dataset

Hierarchy level

dataset Dataset

Other

dataset

Statement

A short-term (four-week) ozone-exposure experiment was conducted in 2014 to investigate the interactive effects of cutting on ozone-induced responses in white clover (Trifolium repens). Seedlings were propagated in early Spring 2014. After three weeks, to introduce a soil microbe, pots were inoculated with a soil slurry mixture. Pots were then divided between 3 treatments: uncut pots (controls), with a single cut to four centimetre (cm)m height immediately prior to ozone exposure and uncut, with a weekly application of Methyl-Jasmonate solution during the experiment. The seedlings were grown on for four weeks then exposed to random ozone profiles for four weeks. A representative quarter of each pot was selected and recordings of healthy, injured or senesced leaves made on in weeks 2, 3 and 4. The harvest of above and below-ground biomass was performed after four weeks on each pot. Shoot biomass, and root biomass from a representative quarter of the pot, were dried for a minimum of 48 hours at 60 degrees Celsius. Root nodule biomass was also excised from the root system, and was air dried, sized and weighed. Root biomass, nodule mass and nodule numbers per pot were calculated. Leaf area index (LAI) of undamaged leaves was determined from dried forage. Mean injury and senescence data across the recordings was determined for each pot, and was arcsine transformed prior to analysis. Injury and senescence data, and selected biomass data, were then analysed by analysis of variance (ANOVA), with ozone background and treatment applied as factors. Post-hoc Tukey's honest significant difference tests were used to determine the significance of factors where appropriate. Relationships between total root nodule biomass and biomass, and raw injury parameters for each treatment were also investigated via Pearson's correlation analysis. All analyses were conducted in R Software (version 3.1.2). Data was first recorded in the field in field notebooks, and was then transferred to electronic copies (MS Excel worksheets). Data was then exported as .csv files for ingestion into the Environmental Information Data Centre.

File identifier

10c6df00-c7ef-444b-951f-33a2c0072bec XML

Metadata language

EnglishEnglish

Character set

ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1

Hierarchy level

dataset Dataset

Hierarchy level name

dataset

Date stamp

2025-11-13T16:22:54

Metadata standard name

UK GEMINI

Metadata standard version

2.3