0bfdfa23-15f8-4ffd-b1dc-e77178af0304 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:21:15 UK GEMINI 2.3 Survival rates and transfer potential measurements of Salmonella enterica Serovar Typhimurium colonising polyethylene microplastics in contaminated agricultural soils 2025-05-02 publication https://catalogue.ceh.ac.uk/id/0bfdfa23-15f8-4ffd-b1dc-e77178af0304 10.5285/0bfdfa23-15f8-4ffd-b1dc-e77178af0304 doi: Woodford, L., White, H., Fellows, R., Ormsby, M., Pow, C., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella enterica Serovar Typhimurium colonising polyethylene microplastics in contaminated agricultural soils. NERC EDS Environmental Information Data Centre 10.5285/0bfdfa23-15f8-4ffd-b1dc-e77178af0304 This dataset contains mesocosm experimental data quantifying the ability of the bacterium Salmonella enterica Serovar Typhimurium (S. Typhimurium) to persist on microplastic beads in podzol and loamy soil. Persistence was determined through measuring surviving colony forming units (CFUs) of S. Typhimurium on the plastics after destructive sampling of the mesocosm jars. The results of a flooding and leachate experiment are also included, containing transfer potential and leachate gradients of S. Typhimurium in each soil under ambient, and flooding conditions. Water was added to soil columns containing the microbeads to simulate flooding, and the CFUs in the water and on virgin plastics were measured to determine transfer potential. In the persistence experiment samples were measured on days 1, 2, 3, 4, 7, 10, 14, 21, 28 and 35, and in the transfer and leachate experiments samples were measured after 7, 14 and 21 days, with four replicates being taken at each time point. This dataset was created as part of a study investigating how human pathogens enter agricultural environments via flooding or contaminated irrigation, and to determine the survivability of S. Typhimurium in these soils. This work is supported by the National Environmental Research Council (NE/V005847/1). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/0bfdfa23-15f8-4ffd-b1dc-e77178af0304 Woodford, L. University of Stirling luke.woodford@stir.ac.uk pointOfContact Woodford, L. University of Stirling luke.woodford@stir.ac.uk https://orcid.org/0000-0003-2530-2120 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author White, H. University of Stirling hannah.white1@stir.ac.uk author Fellows, R. University of Stirling rosie.c.fellows@outlook.com author Ormsby, M. University of Stirling Michael.ormsby@glasgow.ac.uk author Pow, C. University of Stirling chloejean.pow@stir.ac.uk author Quilliam, R. University of Stirling richard.quilliam@stir.ac.uk author University of Stirling EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Human Health and Safety theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Environmental risk Pollution Soil Water quality plastic pollution human pathogens flooding plastisphere leachate salmonella mesocosm experiment otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Woodford, L., White, H., Fellows, R., Ormsby, M., Pow, C., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella enterica Serovar Typhimurium colonising polyethylene microplastics in contaminated agricultural soils. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/0bfdfa23-15f8-4ffd-b1dc-e77178af0304 English utf8 environment 2023-06-01 2023-08-01 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/0bfdfa23-15f8-4ffd-b1dc-e77178af0304 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/0bfdfa23-15f8-4ffd-b1dc-e77178af0304.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Mesocosm experiments were designed in two stages. For the persistence experiment jars were filled with two soil types and microplastic beads were mixed with the soils and left. At each time point destructive sampling was used to recover the beads and measure the surviving CFUs of S. Typhimurium on the plastics. In the flooding and leachate experiment, water was added to columns containing beads and the CFUs recovered in the water was recorded (flooding) and CFUs on virgin plastics was used to measure transfer. In the persistence experiment samples were measured on days 1, 2, 3, 4, 7, 10, 14, 21, 28 and 35, and in the transfer and leachate experiments samples were measured after 7, 14 and 21 days, with four replicates being taken each at each time point.