07c32201-c374-4432-a00b-832dedee571e English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:17:20 UK GEMINI 2.3 OSGB 1936 / British National Grid Fungi and invertebrate environmental DNA detections in soil samples from 21 sites across England, 2023 2024-04-16 publication https://catalogue.ceh.ac.uk/id/07c32201-c374-4432-a00b-832dedee571e 10.5285/07c32201-c374-4432-a00b-832dedee571e doi: Stringer, A., Swan, D. (2024). Fungi and invertebrate environmental DNA detections in soil samples from 21 sites across England, 2023. NERC EDS Environmental Information Data Centre 10.5285/07c32201-c374-4432-a00b-832dedee571e This dataset contains percentages, counts and metrics of fungi, invertebrates, and soil invertebrates from 656 soil samples collected from 21 Forestry England and partner sites across England throughout 2023. The sites included an ancient woodland National Nature Reserve, sitka spruce monocultures, heather moorland and breck heath. Important sites for nature recovery were also sampled: New Forest, Forest of Dean, and Wild Ennerdale. The soil samples were collected as part of the Forestry England 2023 eDNA Survey. The primary aim of the survey was to establish a baseline in order to detect future change alongside reference sites. Full details about this dataset can be found at https://doi.org/10.5285/07c32201-c374-4432-a00b-832dedee571e Stringer, A. Forestry England Andrew.Stringer@ForestryEngland.uk https://orcid.org/0000-0002-6192-275X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Swan, D. NatureMetrics Daniel.Swan@naturemetrics.co.uk https://orcid.org/0000-0001-8978-8129 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Stringer, A. Forestry England Andrew.Stringer@ForestryEngland.uk pointOfContact Forestry England Andrew.Stringer@ForestryEngland.uk owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication fungus invertebrate GEMET - Concepts, version 4.1.3 2019-01-28 creation Environmental survey Soil England metabarcoding Forestry England environmental DNA (eDNA) otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Stringer, A., Swan, D. (2024). Fungi and invertebrate environmental DNA detections in soil samples from 21 sites across England, 2023. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/07c32201-c374-4432-a00b-832dedee571e textTable 15 English utf8 environment biota 2023-01-01 2023-12-31 -6.452 1.768 49.864 55.812 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/07c32201-c374-4432-a00b-832dedee571e Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/07c32201-c374-4432-a00b-832dedee571e.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The soil samples were collected by project officers, district ecologists, and contractors using standard soil sampling approaches and kits provided by NatureMetrics. Nine subsamples were mixed to create a sample. Five samples were taken within a 5ha plot. Between three and ten plots were used per site. Environmental DNA metabarcoding was completed on samples using three primers, ITS2, 18S, and COI by NatureMetrics. NatureMetrics analysed all samples, including their standard quality assurance procedure, and provided the data to Forestry England. Forestry England checked the data and prepared it (e.g., conversion to CSV) for EIDC submission. Please note that several GPS coordinates in the metadata are thought to be inaccurate and are highlighted as such.