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Microbial DNA extraction and PCR from core samples SSK105556 and SSK105560 from UKGEOS Glasgow Observatory, borehole GGC01

Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent Polymerase Chain Reaction (PCR) from core samples collected and preserved for microbiology (SSK105556 and SSK105560) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. NERC grant SHAPE-UK NE/R018006/1.

Simple

Date (Creation)
2023-08-03
Identifier
http://data.bgs.ac.uk/id/dataHolding/13608095
Originator
  University of Bristol - Katie Edwards ( PhD Student School of Earth Sciences )
Wills Memorial Building, Queens Road, Clifton , Bristol , BS8 1RJ ,
Distributor
  British Geological Survey - Enquiries
United Kingdom
Point of contact
  British Geological Survey - Enquiries
United Kingdom
Maintenance and update frequency
notApplicable notApplicable
GEMET - INSPIRE themes, version 1.0
  • Geology
BGS Thesaurus of Geosciences
  • Geomicrobiology
  • Sandstone
  • Shale
dataCentre
  • NGDC Deposited Data
  • Scottish SDI
  • UKGEOS
Keywords
  • NERC_DDC
Access constraints
otherRestrictions Other restrictions
Other constraints
licenceOGL
Other constraints
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
Use constraints
otherRestrictions Other restrictions
Other constraints
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.
Other constraints
There are no restrictions on the use that may be made of the dataset, although an appropriate copyright acknowledgement must be made when any part of the dataset is reproduced. Either no third party data / information is contained in the dataset or BGS has secured written permission from the owner of any third party data / information contained in the dataset to make the dataset freely available without any use constraints - inclusion of any third party data / information will affect the copyright acknowledgement that needs to be made.
Spatial representation type
vector Vector
Metadata language
EnglishEnglish
Topic category
  • Geoscientific information
Geographic identifier
Glasgow [id=1298677]

Date (Revision)
2002

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Begin date
2021-06-01
End date
2021-06-30
Unique resource identifier
OSGB 1936 / British National Grid (EPSG::27700)
Distribution format
  • Microsoft Excel ( witheld )

  • Microsoft Word ( witheld )

Distributor
  British Geological Survey - Enquiries
United Kingdom
OnLine resource
Data ( WWW:DOWNLOAD-1.0-http--download )
Hierarchy level
dataset Dataset
Other
dataset

Conformance result

Date (Publication)
2011
Explanation
See the referenced specification
Pass
No

Conformance result

Date (Publication)
2010-12-08
Explanation
See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF
Pass
No
Statement
Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.
File identifier
027b4472-e717-1de8-e063-0937940a5dd1 XML
Metadata language
EnglishEnglish
Hierarchy level
dataset Dataset
Date stamp
2026-05-06
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  British Geological Survey
Environmental Science Centre,Keyworth , NOTTINGHAM , NOTTINGHAMSHIRE , NG12 5GG , United Kingdom
+44 115 936 3100
Dataset URI
http://data.bgs.ac.uk/id/dataHolding/13608095
 
 

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